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High-performance liquid chromatography of human milk triacylglycerols and gas chromatography of component fatty acids

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Lipids

Abstract

Human milk triacylglycerols were separated by high-performance liquid chromatography. A 5-μ Supelcosil LC-18 column (Supelco, Inc., Bellefonte, PA) was used with acetone/acetonitrile (64∶36, vol/vol) as mobile phase. Triacylglycerols were tentatively identified based on theoretical carbon number and relative retention time. Despite changes resulting from dietary fat variation, the major component triacylglycerols were those composed of palmitic, oleic and linoleic acids. Triacylglycerols with palmitic, stearic and oleic acids were present as minor components. Fatty acids were quantified by gas chromatography relative to an internal standard. Ratios of n−6/n−3 fatty acids were found to be high than previously reported.

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Abbreviations

AA:

arachidonic acid (20∶4n−6)

CN:

carbon number

CV:

coefficient of variation

D:

decanoic acid (10∶0)

DHA:

docosahexaenoic acid (22∶6n−3)

ECN:

equivalent carbon number

EPA:

eicosapentaenoic acid (20∶5n−3)

FA:

fatty acid(s)

FAME:

fatty acid methyl ester(s)

GC:

gas chromatography

HPLC:

high-performance liquid chromatography

L:

lauric acid (12∶0)

LC PUFA:

long-chain polyunsaturated fatty acid(s)

Ln:

linolenic acid (18∶3n−3)

Lo:

linoleic acid (18∶2n−6)

M:

myristic acid (14∶0)

O:

oleic acid (18∶1n−9)

P:

palmitic acid (16∶0)

Po:

palmitoleic acid (16∶1n−7)

TAG:

triacylglycerol

S:

stearic acid (18∶0)

RBC:

red blood cells(s)

TCN:

theoretical carbon number

TLC:

thin-layer chromatography

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Authors and Affiliations

  1. Division of Nutritional Sciences, University of Illinois, Burnsides Research Laboratory, 1208 Pennsylvania Avenue, 61801, Urbana, IL

    Karen D. Dotson & Edward G. Perkins

  2. Department of Food Sciences, University of Illinois Burnsides Research Laboratory, 1208 Pennsylvania Avenue, 61801, Urbana, IL

    John P. Jerrell & Edward G. Perkins

  3. Department of Nutrition, Pennsylvania State University, 16802, University Park, Pennsylvania

    Mary Frances Picciano

Authors
  1. Karen D. Dotson
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  2. John P. Jerrell
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  3. Mary Frances Picciano
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  4. Edward G. Perkins
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Dotson, K.D., Jerrell, J.P., Picciano, M.F. et al. High-performance liquid chromatography of human milk triacylglycerols and gas chromatography of component fatty acids. Lipids 27, 933–939 (1992). https://doi.org/10.1007/BF02535876

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  • DOI: https://doi.org/10.1007/BF02535876

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