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Isolation and analysis of age-related fluorescent substances in rat testes

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Lipids

Abstract

Fluorescent substances were extracted from rat testicular tissue with 2,2-dimethoxypropane (DMP) and analyzed by 2-dimensional thin layer chromatography (TLC). One substance that accumulated with increasing age of the animals was isolated and analyzed quantitatively by spectrophotofluorometry using quinine sulfate as a standard. This substance, which was designated as an age-related fluorescent substance (ARFS), exhibited an excitation maximum at 355 nm and an emission maximum at 490 nm. Its fluorescence was quenched by metal chelators and at alkaline pH, indicating it contained a conjugated Schiff base structure. Quantitative analysis of this substance in the testes of rats 1, 2, 11 and 20 months of age showed that it increased linearly with age. The relation of this substance to aging also was indicated by its detection in animals of different ages fed diets of both low and high unsaturation.

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Author notes

  1. Hiroyuki Shimasaki

    Present address: Department of Biochemistry, Teikyo University School of Medicine, 2-11-1 Itabashi-Ku, Tokyo, Japan

Authors and Affiliations

  1. The Hormel Institute, University ofMinnesota, 55912, Austin, MN

    Hiroyuki Shimasaki & O. S. Privett

  2. Department of Biochemistry, Teikyo University School of Medicine, 2-11-1 Itabashi-Ku, 173, Tokyo, Japan

    Nobuo Ueta

Authors
  1. Hiroyuki Shimasaki
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  2. Nobuo Ueta
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  3. O. S. Privett
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Shimasaki, H., Ueta, N. & Privett, O.S. Isolation and analysis of age-related fluorescent substances in rat testes. Lipids 15, 236–241 (1980). https://doi.org/10.1007/BF02535833

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  • DOI: https://doi.org/10.1007/BF02535833

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