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Bovine pulmonary surfactant: Chemical composition and physical properties

  • Published:
Lipids

Abstract

Bovine pulmonary surfactant was obtained by endotracheal lavage of lungs from newly slaughtered cows followed by differential centrifugation. Lipid extracts of bovine surfactant contained 3% neutral lipid, mainly as cholesterol and diacylglycerol and 97% phospholipid. Phosphatidylcholine (79%) and phosphatidylglycerol (11%) accounted for most of the phospholipids with smaller amounts of phosphatidyl-ethanolamine, phosphatidylinositol, lyso-bis-phosphatidic, acid and sphingomyelin. Fatty acid analysis revealed high levels of palmitate in phosphatidylcholine and to a lesser extent phosphatidylglycerol, but not in the other diacylphospholipids. Phosphatidylcholine was 53% disaturated and phosphatidylglycerol was 23% disaturated. Monoenoic species accounted for the major proportion of the remaining, lipid. The protein content was 10% as estimated by the Lowry procedure and 5% when determined by amino acid analysis. Extraction with chloroform/methanol removed ca. 90% of the protein but had no effect on the surfactant properties as evaluated by a pulsating bubble technique.

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Authors and Affiliations

  1. Department of Obstetrics & Gynaecology, University of Western Ontario, N6A 5A5, London, Ontario, Canada

    Sue Yu, Paul G. R. Harding & Fred Possmayer

  2. Department of Biophysics, University of Western Ontario, N6A 5A5, London, Ontario, Canada

    Norman Smith

Authors
  1. Sue Yu
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  2. Paul G. R. Harding
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  3. Norman Smith
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  4. Fred Possmayer
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Additional information

Some of the results contained in this paper were presented at the 73rd Meeting of the AOCS in Toronto, May, 1982.

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Yu, S., Harding, P.G.R., Smith, N. et al. Bovine pulmonary surfactant: Chemical composition and physical properties. Lipids 18, 522–529 (1983). https://doi.org/10.1007/BF02535391

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