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High pressure liquid chromatographic separation of molecular species of phosphatidic acid dimethyl esters derived from phosphatidylcholine

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Lipids

Abstract

A majority of the individual molecular species of phosphatidic acid dimethyl esters derived from multispecies egg yolk and soybean phosphatidylcholines have been separated by reverse-phase high pressure liquid chromatography. Two Partisil-10 ODS columns connected in tandem and the eluents acetonitrile or methanol/water (95∶5) were used for molecular species resolution, based on total fatty acyl carbon number and degree of unsaturation.

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Authors and Affiliations

  1. Department of Medicinal Chemistry, College of Pharmacy, University of Rhode Island, 02881, Kingston, RI

    John Y-K. Hsieh, David K. Welch & Joseph G. Turcotte

Authors
  1. John Y-K. Hsieh
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  2. David K. Welch
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  3. Joseph G. Turcotte
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Hsieh, J.YK., Welch, D.K. & Turcotte, J.G. High pressure liquid chromatographic separation of molecular species of phosphatidic acid dimethyl esters derived from phosphatidylcholine. Lipids 16, 761–763 (1981). https://doi.org/10.1007/BF02535346

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  • DOI: https://doi.org/10.1007/BF02535346

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