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Activation of rat liver cholesterol ester hydrolase by cAMP-dependent protein kinase and protein kinase C

  • Published:
Lipids

Abstract

Short term regulation of hepatic cholesterol ester hydrolase by reversible phosphorylation is described. Two different kinase systems seem to be involved in this regulation. The addition of ATP, cyclic AMP and Mg2+ to rat liver 104,000× g supernatant (S104) produced a 100–140% increase in cholesterol ester hydrolase activity. This stimulation was abolished when protein kinase inhibitor was added prior to the addition of ATP, cyclic AMP and Mg2+. Cholesterol ester hydrolase activity was also stimulated when calcium ions, phosphatidylserine, and diolein were added to S104 along with ATP and Mg2+. Diolein in this reaction could be substituted by phorbol 12-myristate 13-acetate. Preincubation of S104 with alkaline phosphatase resulted in a deactivation of cholesterol ester hydrolase. The addition of increasing concentrations of Mg2+ to S104 produced increasing inhibition of cholesterol ester hydrolase activity, and this effect was blocked by NaF.

It is suggested that rat liver cholesterol ester hydrolase is activated by cyclic AMP dependent protein kinase and protein kinase C. Deactivation is accomplished by dephosphorylation catalyzed by a phosphoprotein phosphatase, dependent on Mg2+.

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Abbreviations

ACAT:

acylCoA:cholesterol acyltransferase

AMP:

adenosine monophosphate

ATP:

adenosine triphosphate

cAMP:

cyclic 3′,5′-adenosine monophosphate

CEH:

cholesterol ester hydrolase

CEH-P:

phosphorylated form of cholesterol ester hydrolase

DG:

diacylglycerol

PI:

phosphatidylinositol

PKI:

protein kinase inhibitor

TCA:

trichloracetic acid

PMA:

phorbol 12-myristate 13-acetate

S104:

the 104,000×g supernatant

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biophysics, Virginia Commonwealth University, Box 614-MCV Station, Medical College of Virginia, 23298, Richmond, Virginia

    Shobha Ghosh & W. McLean Grogan

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  1. Shobha Ghosh
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  2. W. McLean Grogan
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Ghosh, S., Grogan, W.M. Activation of rat liver cholesterol ester hydrolase by cAMP-dependent protein kinase and protein kinase C. Lipids 24, 733–736 (1989). https://doi.org/10.1007/BF02535213

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  • DOI: https://doi.org/10.1007/BF02535213

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