Abstract
Total lipids from hepatopancreas of the horseshoe crabs,Tachypleus gigas andCarcinoscorpius rotundicauda, obtained in 7.6 and 3.3% wet weight respective yields, were fractionated by various chromatographic techniques and identified by gas-liquid chromatography and spectroscopic methods. Fatty acid-containing lipids were rich in 16∶0 (8.0–25%), 18∶1ω9 (6.9–22%) and 18∶2ω6 (6.8–18.5%); appreciable amounts of 16∶1ω7, 18∶3ω3, 20∶5ω3 and 22∶6ω3 were also present. The level of 26∶0 in the hydrocarbon fractions was unusually high (64 and 68%). Carbon chain lengths of major wax esters were 44, 46 and 48 forT. gigas and 38, 40 and 42 forC. rotundicauda. 1-O-Alkyl diglycerides were 7.2 and 9.1% of the total lipids in the two species and contained 14∶0(20%), 16∶0(60%) and 18∶0(20%) alkyl chains along with a relatively higher percentage (32–35%) of saturated fatty acids. High levels of cholesterol (>50% of total sterol) in the free and combined state were encountered in both samples, phospholipid contents being 40 and 35%, respectively, and contained highest levels of unsaturated fatty acids.
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Abbreviations
- ECL:
-
equivalent chain length
- GLS:
-
gas-liquid chromatography
- NMID:
-
nonmethylene interrupted dienoic
- PL:
-
phospholipids
- RRT:
-
relative retention times
- TAG:
-
triacylglycerol
- TLC:
-
thin-layer chromatography
- TMS:
-
tetramethylsilane
References
Bishayee, S., and ThambiDorai, D. (1980)Biochim. Biophys. Acta 623, 89–97.
Roche, A.C., and Monsígny, M. (1974)Biochim. Biophys. Acta 371, 242–254.
Giese, A.C. (1966)Physiol. Rev. 46, 244–298.
Allen, W.V. (1971)J. Fish Res. Bd. Canada 28, 1191–1195.
Lawrence, J.M. (1971)Crustaceana 4, 264–266.
Jezyk, P.F., and Penicnak, A.J. (1966)Lipids 1, 427–429.
Giese, A.C. (1969)Oceanogr. Mar. Biol. 7, 175–229.
De Moreno, J.E.A., Moreno, V.J., and Brenner, R.R. (1976)Lipids 11, 334–340.
Van der Horst, D.J., Oudejans, R.C.H.M., Plug, A.G., and Van der Sluis, I. (1973)Mar. Biol. 20, 291–296.
Bligh, E.G., and Dyer, W.J. (1959)Can. J. Biochem. Physiol. 37, 911–917.
Rouser, G., Kritchevski, G., and Yamamoto, A. (1967) inLipid Chromatographic Analysis (Marinetti, G.V., ed.) Vol. 1, pp. 118–119, Marcel Dekker, New York.
Mangold, H.K. (1969) inThin Layer Chromatography (Stahl, E., ed.) pp. 363–421, Springer-Verlag, New York.
Dutta A.K., Pal, P.K., Ghosh, A., Misra, S., Nandi, S., and Choudhury, A. (1986)J. Am. Oil Chem. Soc. 63, 223–225.
Mangold, H.K., and Baumann, W.J. (1967) inLipid Chromatographic Analysis (Marinetti, G.V. ed.) Vol. 1, pp. 339–359, Marcel Dekker, New York.
Misra, S., Ghosh, A., and Dutta, J. (1984)J. Sci. Food Agric. 35, 59–65.
Misra, S., Dutta, A.K., Chattopadhyay, S., Choudhury, A., and Ghosh, A. (1987)Phytochemistry 26, 3265–3268.
Misra, S., Choudhury, A., Dutta, A.K., Ghosh, A., and Dutta, J. (1983)J. Chromatogr. 280, 313–320.
Misra, S., Choudhury, A., Dutta, A.K., Dutta, J., and Ghosh, A. (1984)Lipids 19, 302–303.
Misra, S., Choudhury, A., Dutta, A.K., and Ghosh, A. (1984)Phytochemistry 23, 2823–2827.
Schlenk, H., and Gellerman, J.L. (1960)Anal. Chem. 32, 1412–1414.
Hanahan, D.J. (1965)J. Lipid Res., 6, 350–355.
Friedman, L., and Schechter, H. (1960)J. Org. Chem. 25, 877–879.
Smith, G.M., and Djerassi, C. (1987)Lipids 22 236–240.
Kates, M. (1972) inTechniques in Lipidology (Work, T.S., and Work, T.S., eds.) Vol. 3, Part II, pp. 361–364, North Holland, Amsterdam.
Ackman, R.G., and Burgher, R.D. (1965)J. Am. Oil Chem. Soc. 42, 38–42.
Ackman, R.G., Sipos, J.C., and Jangaard, P.M. (1967)Lipids 2, 251–257.
Takagi, T., Kaneniwa, M., Itabashi, Y., and Ackman, R.G. (1986)Lipids 21, 558–565.
Patterson, G.W. (1971)Anal. Chem. 43, 1165–1170.
Knights, B.A. (1973) inModern Methods of Steroid Analysis (Heftmann, E., ed.) pp. 103–108, Academic Press, New York.
Ghosh, A., and Dutta, J. (1972)Trans. Bose Res. Inst. 35, 13–15.
Paradis, M. and Ackman, R.G. (1975)Lipids 10, 12–16.
Paradis, M., and Ackman, R.G. (1977)Lipids 12, 170–176.
Misra, S., Ghosh, M.K., Choudhury, A., Dutta, A.K., Pal, P.K., and Ghosh, A. (1985)J. Sci. Food Agric. 36, 1193–1196.
Misra, S., Misra, S., Choudhury, A., and Ghosh, A. (1986)Food Chem. 22, 251–258.
Moreno, V.J., De Moreno, J.E.A., and Brenner, R.R. (1979)Lipids 14, 15–19.
Sargent, J.R. (1976) inBiochemical and Biophysical Perspectives in Marine Biology, Vol. III (Malines, P.C., and Sargent, J.R., eds.) p. 153, Academic Press, London.
Misra, S., Choudhury, A., and Ghosh, A. (1987)J. Aquor 1, 10–17.
Misra, S., Choudhury, A., Pal, P.K., and Ghosh, A. (1986)Phytochemistry 25, 1083–1087.
Misra, S., Choudhury, A., Chattopadhyay, S., and Ghosh, A. (1988)Phytochemistry 27, 361–364.
Reinhardt, S.B., and Van Vleet, E.S. (1986)Mar. Biol. (Berlin) 91, 149–159.
Djerassi, C., Theobold, N., Kokke, W.C.M.C., Pak, C.S., and Carlson, R.M.K. (1979)Pure Appl. Chem. 51, 1815–1828.
Wood, R., and Snyder, F. (1967)J. Lipid. Res. 8, 494–500.
Mangold, H.K., and Weber, N. (1987)Lipids 22, 789–799.
Ratnayake, W.M.N., Timmins, A., Oshima, T., and Ackman, R.G. (1986)Lipids 21, 518–524.
Snyder, F. (1969) inPrgor. Chem. Fats Other Lipids (Holman, R.T., ed.) Vol. 10, pp. 287–335, Pergamon Press, Oxford.
Snyder, F., ed., (1972)Ether Lipids: Chemistry and Biology, Academic Press, New York.
Mangold, H.K. (1979)Angew. Chem. Int. Ed. Engl. 18, 493–503.
Mangold, H.K., and Paltauf, F., eds. (1983)Ether Lipids: Biochemical and Biomedical Aspects, Academic Press, New York.
Hallgren, B. (1983) inEther Lipids: Biochemical and Biomedical Aspects (Mangold, H.K., and Paltauf, F., eds.) pp. 261–275, Academic Press, New York.
Berdel, W.E., Fink, U., Fromm, M., Egger, B., Reichert, A., Zanker, K.S., and Rastetter, J. (1985) inThe Pharmacological Effect of Lipids II (Kabara, J.J., ed.) pp. 273–279, American Oil Chemists' Society, Champaign.
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Pakrashi, S.C., Duffa, P.K., Achari, B. et al. Lipids and fatty acids of the horseshoe crabsTachypleus gigas andCarcinoscorpius rotundicauda . Lipids 24, 443–447 (1989). https://doi.org/10.1007/BF02535154
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DOI: https://doi.org/10.1007/BF02535154