Abstract
Neonatal mice given nontoxic dermal applications of an industrial surfactant, Toximul MP8 (Tox), and subsequently infected with sublethal doses of mouse-adapted human Influenza B (Lee) virus (FluB) develop many of the biochemical features of Reye's Syndrome (RS). To determine whether these also include abnormal circulating lipid, we examined serum lipid profiles in the mouse model throughout the treatment course using Iatroscan-TH10. Following 10 days of exposure to surfactant, serum phospholipid and cholesterol levels were significantly reduced relative to control animals. These reductions were transient; however, four days following virus administration, significant differences in serum lipid were again evident. These abnormalities coincided and correlated with increased animal mortality. Animals that received combined Tox + virus treatment had significant decreases in serum total lipids relative to control animals, a reflection of a reduction in all lipid classes, including phospholipid, cholesterol, neutral glycerides (triglycerides plus diglycerides) and free fatty acids. Phospholipid (specifically phosphatidylcholine and lysophosphatidylcholine) and free fatty acid levels in the Tox + virus group were also significantly lower than those in animals that received virus alone. This study has demonstrated that suckling mice given chemical/viral treatment have the serum hypopanlipidemia but not the freefattyacidemia that are characteristic of RS.
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Pollack J.D., Cramblett, H.G., Flynn, D., and Clark, D. (1975) inReye's Syndrome (Pollack, J.D., ed.) pp. 227–243, Grune and Stratton, New York.
Ogburn, P.L. Jr. (1976)South. Med. J. 69, 632–633.
Mamunes, P., Devries, G.H., Miller, C.D., and David, R.B. (1975) inReye's Syndrome (Pollack, J.D., ed.) pp. 245–259, Grune and Stratton, New York.
Trauner, D.A., Nyhan, W.L., and Sweetman, L. (1975)Neurology 25, 296–298.
Chaves-Carballo, E., Ellefson, R.D., and Gomez, M.R. (1976)Mayo Clin. Proc. 51, 770–776.
Delong, G.R., and Glick, T.H. (1982)Pediatrics 69, 53–63.
Kang, E.S., Gates, R.E., and Wrenn, E.L. Jr. (1982)Biochem. Med. 27, 180–194.
Zammit, V.A. (1983)Proc. Nutr. Soc. 42, 289–302.
Ng, K.J., Andresen, B.D., Hilty, M.D., and Bianchine, J.R. (1983)J. Chromatogr. 276, 1–10.
Bourgeois, C.H., Shank, R.C., Grossman, R.A., Johnsen, D.O., Wooding, W.L., and Chandavimol, P. (1971)Lab. Invest. 24, 206–216.
Glasgow, A.M., and Chase, H.P. (1975)Pediatr. Res. 9, 133–138.
Deshmukh, D.R., Maassab, H.F., and Mason, M. (1982)Proc. Natl. Acad. Sci. USA 79, 7557–7560.
Deshmukh, D.R., Deshmukh, G.D., Shope, T.C., and Radin, N.S. (1983)Biochim. Biophys. Acta 753, 153–158.
Yoder, M.C., Egler, J.M., Yudkoff, M., Chatter, J., Douglas, S.D., and Polin, R.A. (1985)Infect. Immun. 47, 329–331.
Chaves-Carballo, E., Carter, G.A., and Wiebe, D.A. (1979)Pediatrics 64, 592–597.
Crocker, J.F.S., Renton, K.W., Lee, S.H., Rozee, K.R., Digout, S.C., and Malatjalian, D.A. (1986)Lab. Invest. 54, 32–40.
Lee, S.H.S., Lalto, M., Crocker, J.F.S., and Rozee, K.R. (1980)Appl. Environ. Microbiol. 40, 787–793.
Ackman, R.G. (1981) inMethods in Enzymology (Lowenstein, J.M., ed.) Vol. 72, pp. 205–252, Academic Press, New York.
Folch, J., Lees, M., and Sloane-Stanley, G.H. (1957)J. Biol. Chem. 226, 497–506.
Parrish, C.C., and Ackman, R.G. (1985)Lipids 20, 521–530.
Trauner, D.A. (1982)Pediatr. Res. 16, 950–953.
Kang, E.S., Olson, G., Jabbour, J.T., Solomon, S.S., Heinberg, M., Sabesin, S., and Griffith, J.F. (1984)Proc. Natl. Acad. Sci. USA 81, 6169–6173.
Couch, R.B. (1985) inReye's Syndrome IV (Pollack, J.D., ed.) pp. 86–99, National Reye's Syndrome Foundation, Bryan, Ohio.
Linneman, C.C., Veda, K., Hug, G., Schaeffer, A., Clark, A., and Schiff, G.M. (1979)Pediatr. Res. 13, 44–47.
Hug, G., Bosken, J., Bove, K., Linnemann, C.C., and McAdames, L. (1981)Lab. Invest. 45, 89–109.
Davis, L.E., Cole, L.L., Lockwood, S.J., and Kornfeld, M. (1983)Lab. Invest. 48, 140–147.
MacDonald, M.G., McGrath, P.P., McMartin, D.N., Washington, G.C., and Hudak, G. (1984)Pediatr. Res. 18, 181–187.
Ogburn, P.L. Jr., Sharp, H., Lloyd-Still, J.D., Johnson, S.B., and Holman, R.T. (1982)Proc. Natl. Acad. Sci. USA 79, 908–911.
Grossberg, S.E., Frerman, F.E., and Lengle, E.E. (1972)Immunobiol. Stand. 5, 279–293.
Blough, H.A., and Tiffany, J.M. (1973)Adv. Lipid Res. 11, 267–339.
Campbell, A.E., Loria, R.M., Madge, G.E., and Kaplan, A.M. (1982)Infect. Immun. 37, 307–317.
Garry, R.F., Bostick, D.A., Schram, R., and Waite, M.R.F. (1985)J. Gen. Virol. 66, 1171–1177.
May, H.E., and McCay, P.B. (1968)J. Biol. Chem. 243, 2288–2295.
Brown, R.E., and Foreman, D.T. (1982)CRC Crit. Rev. Clin. Lab. Sci. 17, 247–297.
Beisel, W.R., and Fiser, R.H. Jr. (1970)Amer. J. Clin. Nutr. 23, 1069–1079.
Bruell, J.H., Daroczy, A.F., and Hellerstein, H.K. (1962)Science 135, 1071–1072.
Yamamoto, R.S., Crihenden, L.B., Sokoloff, L., and Jay, G.E. (1963)J. Lipid Res. 4, 413–418.
Cuendet, G.S., Loten, E.G., Cameron, D.P., Renold, A.E., and Marliss, E.B. (1975)Amer. J. Physiol. 228, 276–283.
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Murphy, M.G., Archambault-Schertzer, L., Ackman, R.G. et al. Serum lipid abnormalities in a chemical/viral mouse model for Reye's syndrome. Lipids 21, 378–382 (1986). https://doi.org/10.1007/BF02534931
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DOI: https://doi.org/10.1007/BF02534931