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Stereospecificity of different lipases

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Lipids

Abstract

The stereospecificity of 4 lipases towards enantiomeric alkyldiacylglycerols and alkylmonoacylglycerols was investigated. No stereospecific breakdown of the former substrate was observed for lipases from pancreas,Rhizopus arrhizus, Pseudomonas fluorescens, or bile salt-stimulated lipase from human milk. All lipases degraded 2-oleoyl-3-tetradecyl-sn-glycerol faster than 1-tetradecyl-2-oleoyl-sn-glycerol. Among X-1-acyl-3-alkylglycerol isomers, 1-acyl-3-alkyl-sn-glycerol was preferentially attacked by the 3 first mentioned lipases. Possible mechanisms and metabolic implications for these stereospecificities are discussed.

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Authors and Affiliations

  1. Department of Clinical Chemistry, Chemical Center, Stockholm, Sweden

    B. Akesson

  2. Division of Organic Chemistry 1, Chemical Center, Stockholm, Sweden

    S. Gronowitz & P. Michelsen

  3. Vitrum AB, University of Lund, Stockholm, Sweden

    B. Herslöf

  4. Department of Chemistry, Section on Physiological Chemistry, University of Umea, Sweden

    T. Olivecrona

Authors
  1. B. Akesson
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  2. S. Gronowitz
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  3. B. Herslöf
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  4. P. Michelsen
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  5. T. Olivecrona
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Additional information

A preliminary account of this work was given in Abstract 457 of the ISF/AOCS World Congress, New York, 1980.

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Akesson, B., Gronowitz, S., Herslöf, B. et al. Stereospecificity of different lipases. Lipids 18, 313–318 (1983). https://doi.org/10.1007/BF02534708

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  • DOI: https://doi.org/10.1007/BF02534708

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