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Study of free and bound lipids ofBrassica campestris, var yellow sarson

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Lipids

Abstract

Mature seeds ofBrassica campestris var. yellow sarson were extracted with hexane to yield free lipid. The residue then was extracted with chloroform-methanol to release bound lipid. Free and bound lipids were separated into polar and nonpolar fractions chromatographically. The nonpolar fraction of both free and bound lipid consisted mainly of triglycerides with small amounts of steryl esters, free sterols, mono- and di-glycerides, and free fatty acids. The principal components of polar bound lipid were phosphatidyl choline, phosphatidyl ethanolamine, phosphatidyl inositol, and steryl glycoside. In the free polar lipid, there was more phosphatidyl inositol and less phosphatidyl choline and phosphatidyl ethanolamine. Erucic acid content was much greater in the nonpolar fractions and in the polar free lipid than in the polar bound lipid.

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Authors and Affiliations

  1. Food Research Institute, Canada Agriculture, K1A OC6, Ottawa, Canada

    Mary E. McKillican & J. A. G. Larose

Authors
  1. Mary E. McKillican
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  2. J. A. G. Larose
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Contribution No. 218 from the Food Research Institute, Canada Department of Agriculture, Ottawa, Canada.

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McKillican, M.E., Larose, J.A.G. Study of free and bound lipids ofBrassica campestris, var yellow sarson. Lipids 9, 455–460 (1974). https://doi.org/10.1007/BF02534271

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  • DOI: https://doi.org/10.1007/BF02534271

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