Skip to main content
SpringerLink
Log in

Antioxidants in neoplastic cells: I. Changes in the antioxidative capacity of mouse neuroblastoma cells measured by a single-phase assay

  • Published:
Lipids

Abstract

Cultured mouse neuroblastoma cells exhibit a striking increase in antioxidative capacity during the transition from logarithmically dividing cells to nondividing, neurite-bearing cells. Two physically separable phenomena are involved: (a) the membrane pellet of neurite-bearing cells is highly resistant to lipid peroxidation, and (b) the postmicrosomal supernatant of these cells inhibits peroxidation in rat liver mitochondria and other biological membranes. A precise, single-phase assay has been developed for assessing antioxidant levels in lipid extracts. By means of this assay, the increase in membrane resistance to lipid peroxidation has been correlated with a threefold increase in the antioxidant activity of the neuroblastoma neutral lipid fraction. This finding implies that generation of a neutral lipid antioxidant (or antioxidants) is involved in the profound increase in antioxidative capacity which occurs in differentiating neuroblastoma cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Barber, A.A., and F. Bernheim, in “Advances in Gerontological Research,” edited by B.L. Strehler, Vol. 2, Academic Press, New York, 1967, p. 355.

    Google Scholar 

  2. Kohn, H.I., and M. Liversedge, J. Pharmacol. Exp. Ther., 82:292 (1944).

    CAS  Google Scholar 

  3. Donnan, S.K., J. Biol. Chem., 182:915 (1950).

    Google Scholar 

  4. Shuster, C.W., Proc. Soc. Exp. Biol. Med., 90:423 (1955).

    PubMed  CAS  Google Scholar 

  5. Thiele, E.H., and J.W. Huff, Arch. Biochem. Biophys., 88:208 (1960).

    Article  PubMed  CAS  Google Scholar 

  6. Thiele, E.H., and J.W. Huff, Proc. Soc. Exp. Biol. Med., 118:689 (1965).

    PubMed  CAS  Google Scholar 

  7. Lash, E.D., Arch. Biochem. Biophys., 115:332 (1966).

    Article  PubMed  CAS  Google Scholar 

  8. Utsumi, K., G. Yamamoto and K. Inaba, Biochim. Biophys. Acta, 105:368 (1965).

    PubMed  CAS  Google Scholar 

  9. Utsumi, K., Y. Kanemasa, T. Yoshioka, and T. Oda, Life Sci., 9(II):473 (1970).

    Article  CAS  Google Scholar 

  10. Utsumi, K., N. Goto, Y. Kanemasa, T. Yoshioka, and T. Oda, Physiol. Chem. Phys., 3:468 (1971).

    Google Scholar 

  11. Bernheim, F., A. Ottolenghi, and K.M. Wilbur, Radiat. Res., 4:132 (1956).

    Article  PubMed  CAS  Google Scholar 

  12. Wolfson, N., K.M. Wilbur, and F. Bernheim, Exp. Cell Res., 10:556 (1956).

    Article  PubMed  CAS  Google Scholar 

  13. Tappel, A.L., Ann. N.Y. Acad. Sci., 203:12 (1972).

    PubMed  CAS  Google Scholar 

  14. Plaa, G.L., and H. Witschi, Ann. Rev. Pharmacol. 16:125 (1976).

    Article  CAS  Google Scholar 

  15. Prasad, K.N., Biol. Rev., 50:129 (1975).

    PubMed  CAS  Google Scholar 

  16. Augusti-Tocco, G., and G. Sato, Proc. Natl. Acad. Sci. USA, 64:311 (1969).

    Article  PubMed  CAS  Google Scholar 

  17. Foulds, L., “Neoplastic Development,” Vol. 1, Academic Press, New York, 1969, p. 318.

    Google Scholar 

  18. Arneson, R.M., and J.D. Wander, Lipids, 13:391 (1978).

    PubMed  CAS  Google Scholar 

  19. Blume, A., F. Gilbert, S. Wilson, J. Farber, R. Rosenberg, and M. Nirenberg, Proc. Natl. Acad. Sci. USA, 67:786 (1970).

    Article  PubMed  CAS  Google Scholar 

  20. Paul, J., “Cell and Tissue Culture,” 4th Edition, E. and S. Livingstone, London, 1970, p. 218.

    Google Scholar 

  21. Phillips, H.J., in “Tissue Culture, Methods and Applications,” Edited by P.F. Kruse, Jr., and M.K. Patterson, Jr., Academic Press, New York, 1973, p. 406.

    Google Scholar 

  22. Schubert, D., H. Tarikas, A.J. Harris, and S. Heinemann, Nature New Biol., 223:79 (1971).

    Google Scholar 

  23. McGarrity, G.J., Tissue Culture Association Manual, 1:113 (1975).

    Article  Google Scholar 

  24. Lowry, O.H., N.J. Rosebrough, A.L. Farr, and R.J. Randall, J. Biol. Chem., 193:265 (1951).

    PubMed  CAS  Google Scholar 

  25. Ottolenghi, A., Arch. Biochem. Biophys., 79:355 (1959).

    Article  CAS  Google Scholar 

  26. Placer, Z.A., L.L. Cushman, and B.C. Johnson, Anal. Biochem., 16:359 (1966).

    Article  PubMed  CAS  Google Scholar 

  27. Wills, E.D., Biochim. Biophys. Acta, 84:475 (1964).

    PubMed  CAS  Google Scholar 

  28. Johnson, D., and H. Lardy, Methods Enzymol. 10:94 (1967).

    Article  CAS  Google Scholar 

  29. May, H.E., and P.B. McCay, J. Biol. Chem. 243:2288 (1968).

    PubMed  CAS  Google Scholar 

  30. Avis, P.J.G., in “Subcellular Components, Preparation and Fractionation,” Edited by G.D. Birnie, 2nd Edition, University Park Press, Baltimore, MD, 1972, p. 1.

    Google Scholar 

  31. Bligh, E.G., and W.J. Dyer, Can. J. Biochem. Physiol., 37:911 (1959).

    PubMed  CAS  Google Scholar 

  32. Kates, M., “Techniques in Lipidology,” American Elsevier Publishing Co., New York, 1972, p. 393.

    Google Scholar 

  33. Mair, R.D., and R.T. Hall, in “Treatise on Analytical Chemistry,” Edited by I.M. Kolthoff and P.J. Elving, Part II Vol. 1, John Wiley and Sons, New York, 1971, p. 420.

    Google Scholar 

  34. Rogers, S., and P. Rous, J. Exp. Med., 93:459 (1951).

    Article  PubMed  CAS  Google Scholar 

  35. Sharma, R.K., and K. Hashimoto, Cancer Res. 32:666 (1972).

    PubMed  CAS  Google Scholar 

  36. Papageorgiou, P.S., W.L. Henley, and P.R. Glade, J. Immunol., 108:494 (1972).

    PubMed  CAS  Google Scholar 

  37. Hatefi, Y., and W.G. Hanstein, Arch. Biochem. Biophys., 138:87 (1970).

    Article  PubMed  Google Scholar 

  38. Kellogg, E.W., III, and I. Fridovich, J. Biol. Chem. 250:8812 (1975).

    PubMed  CAS  Google Scholar 

  39. Pryor, W.A., “Free Radicals,” McGraw-Hill Book Co., New York, 1966, p. 128.

    Google Scholar 

  40. Struve, W.G., R.M. Arneson, J.E. Chenevey, and C.K. Cartwright, Exp. Cell. Res., 109:381 (1977).

    Article  PubMed  CAS  Google Scholar 

  41. Hoekstra, W.G., Fed. Proc. Am. Soc. Exp. Biol. 34:2083 (1975).

    CAS  Google Scholar 

  42. Germain, G.S., and R.M. Arneson, Biochem. Biophys. Res. Commun., 79:119 (1977).

    Article  PubMed  CAS  Google Scholar 

  43. Williams, M.L., Biol. Neonate, 23:25 (1973).

    Article  PubMed  CAS  Google Scholar 

  44. Kehrer, J.P., and A.P. Autor, Arch. Biochem. Biophys., 181:73 (1977).

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, The University of Tennessee Center for the Health Sciences, 38163, Memphis, Tennessee

    Richard M. Arneson, Vincent J. Aloyo, Glen S. Germain & James E. Chenevey

Authors
  1. Richard M. Arneson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Vincent J. Aloyo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Glen S. Germain
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. James E. Chenevey
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Arneson, R.M., Aloyo, V.J., Germain, G.S. et al. Antioxidants in neoplastic cells: I. Changes in the antioxidative capacity of mouse neuroblastoma cells measured by a single-phase assay. Lipids 13, 383–390 (1978). https://doi.org/10.1007/BF02533706

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02533706

Keywords

Search

Navigation