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High pressure reverse phase liquid chromatography of fatty acidp-bromophenacyl esters

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Lipids

Abstract

High pressure reverse phase liquid chromatography has been employed to rapidly separate saturated and unsaturated fatty acids as the correspondingp-bromophenacyl esters. Through the use of a highly efficient C18 reverse phase column packing, it has also been possible to distinguish among geometrical and positional isomers of the unsaturated acids. The use of ultroviolet-sensitive esters has permitted the detection of low (nanogram range) concentrations of fatty acids. The time required for analysis has been further reduced by employing a novel and rapid method for the preparation of the esters.

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Pel, P.T.S., Kossa, W.C., Ramachandran, S. et al. High pressure reverse phase liquid chromatography of fatty acidp-bromophenacyl esters. Lipids 11, 814–816 (1976). https://doi.org/10.1007/BF02533409

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  • DOI: https://doi.org/10.1007/BF02533409

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