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Biosynthesis of prostaglandins

  • Published:
Lipids

Abstract

Highly purified cyclooxygenase from sheep vesicular glands is stimulated by the presence of protoporphyrin IX compounds. This stimulation may be due to the conversion of an apoenzyme to the holoenzyme, and full activity is achieved when half of the enzyme subunits (70,000 daltons) bind heme. Also, one-half of the subunits appear to contain non-heme iron. The apparent molecular weight of the holoenzyme is approximately 300,000 daltons and is compatible with a complex of four 70,000 dalton subunits. Thus, we suggest that heme and non-heme iron may be attached to different 70,000 daltons subunits that make up an A2B2-type of peptide chain arrangement.

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References

  1. Cook, H.W. and W.E.M. Lands, Biochem. Biophys. Res. Commun. 65:464 (1975).

    Article  PubMed  CAS  Google Scholar 

  2. Smith, W.L., and W.E.M. Lands, Biochemistry 11:3276 (1972).

    Article  PubMed  CAS  Google Scholar 

  3. Cook, H.W., and W.E.M. Lands, Nature 260:630 (1976).

    Article  PubMed  CAS  Google Scholar 

  4. Rome, L.H., and W.E.M. Lands, Proc. Nat. Acad. Sci. U.S.A. 72:4862 (1975).

    Google Scholar 

  5. Lands, W.E.M., H.W. Cook, and L.H. Rome in “Advances in Prostaglandin and Thromboxane Research”, Vol. I, Edited by B. Samuelsson and R. Paoletti, Raven Press, New York, NY 1976, p. 7.

    Google Scholar 

  6. Smith, W.L., Prostaglandins 10:983 (1975).

    Article  PubMed  CAS  Google Scholar 

  7. Hemler, M., W.E.M. Lands, and W.L. Smith, J. Biol. Chem. 251:5575 (1976).

    PubMed  CAS  Google Scholar 

  8. Lowry, O.H., N.J. Rosebrough, A.L. Farr, and R.J. Randall, J. Biol. Chem. 193:265 (1953).

    Google Scholar 

  9. Falk, J.E., “Porphyrins and Metalloporphyrins”, Elsevier Publishing Co., New York, NY, 1965, p. 181, 240.

    Google Scholar 

  10. Rome, L.H., and W.E.M. Lands, Prostaglandins 10:813 (1975).

    PubMed  CAS  Google Scholar 

  11. Smith, W.L., Prostaglandins (In Press).

  12. Miyamoto, T., N. Ogino, S. Yamamoto, and O. Hayaishi, J. Biol. Chem. 251:2629 (1976).

    PubMed  CAS  Google Scholar 

  13. Yoshimoto, A., I. Hiroyuki, and T. Kenkichi, J. Biochem. 68:487 (1970).

    PubMed  CAS  Google Scholar 

  14. Rosa, M., and A. Francke, Biochim. Biophys. Acta 327:24 (1973).

    Google Scholar 

  15. Schutz, G., and P. Feigelson, J. Biol. Chem. 247:5327 (1972).

    PubMed  CAS  Google Scholar 

  16. Feigelson, P., and F.O. Brady, in “Molecular Mechanisms of Oxygen Activation”, Edited by O. Hayaishi, Academic Press, New York, NY, 1974, p. 87.

    Google Scholar 

  17. Hirata, F., and O. Hayaishi, J. Biol. Chem. 250:5960 (1975).

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biological Chemistry, The University of Michigan, 48109, Ann Arbor, MI

    Martin E. Hemler & William E. M. Lands

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  1. Martin E. Hemler
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  2. William E. M. Lands
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Hemler, M.E., Lands, W.E.M. Biosynthesis of prostaglandins. Lipids 12, 591–595 (1977). https://doi.org/10.1007/BF02533387

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  • DOI: https://doi.org/10.1007/BF02533387

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