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Incorporation of 1-14C-Acetate into C26 fatty acids of the marine spongeMicrociona prolifera

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Lipids

Abstract

The incorporation of 1-14C-acetate into the many fatty acids of the marine spongeMicrociona prolifera was investigated. Probable precursors of 26∶2Δ5,9 and 26∶3Δ5,9,19 showed high levels of radioactivity, supporting the following pathways for the biosynthesis of C26 acids:

$$\begin{array}{*{20}c} {16:0 \to \to \to 26:0 \to 26:1\Delta 9 \to 26:2\Delta 5,9} \\ {16:1\Delta 9 \to \to \to 26:1\Delta 19 \to } \\ {26:2\Delta 9,19 \to 26:3\Delta 5,9,19} \\ \end{array}$$

Degradation of the unsaturated C26 acids at their double bonds showed that the14C was concentrated near the carboxyl end of the chain. Hence, chain elongation was the major mechanism for acetate incorporation into these acids.

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References

  1. Jefferts, E., R.W. Morales, and C. Litchfield, Lipids 9:244 (1974).

    PubMed  CAS  Google Scholar 

  2. Morales, R.W., and C. Litchfield, Biochim. Biophys. Acta 431:206 (1976).

    PubMed  CAS  Google Scholar 

  3. Humphreys, T., Develop. Biol. 8:27 (1963).

    Article  CAS  Google Scholar 

  4. Niederl, J., and V. Niederl, “Micromethods of Quantitative Organic Analysis,” Wiley, New York, NY, 1942, pp. 69–78.

    Google Scholar 

  5. Folch, J., M. Lees, and G.H.S. Stanley, J. Biol. Chem. 226:497 (1957).

    PubMed  CAS  Google Scholar 

  6. Johnston, P.V., “Basic Lipid Methodology,” University of Illinois Press, Urbana, IL, 1971, p. 76.

    Google Scholar 

  7. Privett, O.S., and E.C. Nickell, JAOCS 39:414 (1962).

    CAS  Google Scholar 

  8. Smith Jr., C.R., M.O. Bagby, T.K. Miwa, R.L. Lohmar, and I.A. Wolff, J. Org. Chem. 25:1770 (1960).

    Article  CAS  Google Scholar 

  9. Haverkamp-Begemann, P., and K. de Jong, Rec. Trav. Chim. 78:275 (1959).

    Article  Google Scholar 

  10. Privett, O.S., and E.C. Nickell, Lipids 1:98 (1966).

    Article  CAS  PubMed  Google Scholar 

  11. Litchfield, C., and R.W. Morales, in “Aspects of Sponge Biology,” Edited by F.W. Harrison and R.R. Cowden, Academic Press, New York, NY, 1976, pp. 183–200.

    Google Scholar 

  12. Volpe, J.J., and P.R. Vagelos, Annu. Rev. Biochem. 42:21 (1973).

    Article  PubMed  CAS  Google Scholar 

  13. Stumpf, P.K., Ibid. 38:159 (1969).

    Article  PubMed  CAS  Google Scholar 

  14. Brett, D., D. Howling, L.J. Morris, and A.T. James, Arch. Biochem. Biophys. 143:535 (1971).

    Article  PubMed  CAS  Google Scholar 

  15. Brenner, R.R., Lipids 6:567 (1971).

    Article  PubMed  CAS  Google Scholar 

  16. Goldberg, I., I. Shechter, and K. Bloch, Science 182:497 (1973).

    Article  PubMed  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Biochemistry, Rutgers University, 08903, New Brunswick, NJ

    Reginald W. Morales & Carter Litchfield

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  1. Reginald W. Morales
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  2. Carter Litchfield
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Morales, R.W., Litchfield, C. Incorporation of 1-14C-Acetate into C26 fatty acids of the marine spongeMicrociona prolifera . Lipids 12, 570–576 (1977). https://doi.org/10.1007/BF02533383

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  • DOI: https://doi.org/10.1007/BF02533383

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