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Identification of the free and conjugated sterol in a non-photosynthetic diatom,Nitzschia alba, as 24-methylene cholesterol

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Lipids

An Erratum to this article was published on 01 May 1978

Abstract

Previous studies on the sterol fraction of the nonphotosynthetic marine diatom,Nitszchia alba, indicated the major sterol to be either brassicasterol (24R-methylcholesta-5,22-dien-3β-ol) or 22-dehydrocampesterol (24S-methylcholesta-5,22-dien-3β-ol) on the basis only of gas chromatographymass spectral analysis. The present studies using nuclear magnetic resonance, infrared, and gas chromatography-mass spectrometry on the free and bound sterol fractions isolated by preparative thin layer chromatography showed the presence in both fractions of a single sterol, with spectral and chromatographic properties identical with those reported for 24-methylenecholesterol (ergosta-5,24(28)-dien-3β-ol). This sterol may be the precursor of 24-methyl sterols found in diatoms. The bound sterol fraction was found to consist of a single compound identified as 24-methylenecholesterol sulfate. No sterol esters or sterol glycosides were detected.

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Author notes

  1. R. Anderson

    Present address: Department of Bacteriology and Immunology, University of Western Ontario, London, Canada

Authors and Affiliations

  1. Department of Biochemistry, University of Ottawa, K1N 6N5, Ottawa, Canada

    M. Kates, P. Tremblay & R. Anderson

  2. Scripps Institution of Oceanography, University of California, San Diego, 92093, La Jolla, California

    B. E. Volcani

Authors
  1. M. Kates
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  2. P. Tremblay
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  3. R. Anderson
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  4. B. E. Volcani
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Additional information

An erratum to this article is available at http://dx.doi.org/10.1007/BF02533729.

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Kates, M., Tremblay, P., Anderson, R. et al. Identification of the free and conjugated sterol in a non-photosynthetic diatom,Nitzschia alba, as 24-methylene cholesterol. Lipids 13, 34–41 (1978). https://doi.org/10.1007/BF02533364

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  • DOI: https://doi.org/10.1007/BF02533364

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