Abstract
Microscopic and analytical studies of the lipids in the heart and muscle of the BIO 82.62 myopathic hamsters and agematched normal animals at the average ages of 33, 67, and 108 days were performed. Microscopic examinations did not show increased lipid depositions in the hearts of the diseased animals as was found in the BIO 14.6 strain. No consistent differences in the lipid content of the cardiomyopathic hamsters (BIO 82.62) and age-matched controls were observed in the three age groups except in the cholesterol content of muscle. Cholesterol increased significantly (P<0.01) in the 67 and 108 day old animals. This increase elevated the cholesterol/phospholipid ratio. Analysis of the fatty acid composition of triglycerides showed that the cardiomyopathic hamsters store more saturated fatty acids in both heart and muscle than do their normal counterparts. The abundance of more saturated fatty acids may imply that either the desaturation mechanism is altered in the diseased animals or that unsaturated fatty acids are preferentially utilized in other processes.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Homburger, F., and E. Bajusz, J. Am. Med. Assoc. 212:604 (1970).
Bajusz, E., J.R. Baker, C.W. Nixon, and F. Homburger, Ann. N.Y. Acad. Sci. 156:105 (1969).
Owens, K., W.B. Weglicki, E.H. Sonnenblick, and E.W. Gertz, J. Mol. Cell. Cardiol. 4:229 (1972).
Owens, K., W.B. Weglicki, W.B. Ruth, R.C. Stam, and E.H. Sonnenblick, Biochim. Biophys. Acta 296:71 (1973).
Sulakhe, P.V., and N.S. Dhalla, J. Clin. Invest. 50:1019 (1971).
Lochner, A., L. Opie, A.J. Brink, and A.R. Bosman, Cardiovasc. Res. 2:297 (1968).
Lindenmayer, G.E., S. Harigaya, E. Bajusz, and A. Schwartz, J. Mol. Cell. Cardiol. 1:249 (1970).
Bajusz, E., Am. Heart J. 77:686 (1969).
Kako, K.J., M.J. Thornton, and H.A. Heggtveit, Circ. Res. 34:570 (1974).
Paterson, R.A., R.A. Layberry, and B.B. Nadkarni, Lab. Invest. 26:755 (1972).
Barakat, H.A., G.L. Dohm, and S.N. Pennington, Life Sci. 17:1069 (1975).
Dohm, G.L., H.A. Barakat, T.P. Stephenson, E.B. Tapscott, and S.N. Pennington, Ibid. 17:1075 (1975).
Amenta, J.S., J. Lipid Res. 5:270 (1964).
Freeman, C.P., and D. West, ibid. 7:324 (1966).
Frings, C.S., and R.T. Dunn, Am. J. Clin. Pathol. 53:89 (1970).
Tapscott, E.B., and G.L. Dohm, J. Chromatogr. 107:420 (1975).
Bartlett, G.R., J. Biol. Chem. 234:466 (1959).
Kankare, P., and O. Souvaniemi, J. Chromatogr. 62:485 (1971).
Ast, H.J., Anal. Chem. 35:1539 (1963).
Metcalfe, L.D., A.A. Schmitz and J.R. Pelka, Ibid. 38:514 (1966).
Owens, K., and B.P. Hughs, J. Lipid Res. 11:486 (1970).
Borowski, I.F.M., J.A.C. Harrow, E.T. Pritchard, and N.S. Dhalla, Res. Commun. Chem. Pathol. Pharmacol. 7:443 (1974).
Schefler, W.C., “Statistics for the Biological Sciences,” Addison-Wesley Inc., Reading, MA, 1969, p. 112.
Weiner, B.J., “Statistical Principles in Experimental Design,” 2nd Edition, McGraw Hill, New York, NY, 1971, p. 216.
Author information
Authors and Affiliations
About this article
Cite this article
Barakat, H.A., Lynis Dohm, G., Loesche, P. et al. Lipid content and fatty acid composition of heart and muscle of the BIO 82.62 cardiomyopathic hamster. Lipids 11, 747–751 (1976). https://doi.org/10.1007/BF02533049
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02533049