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Low temperature direct methylation of lipids in biological materials

  • Published:
Lipids

Abstract

The procedure for low temperature methylation of fatty acids in lipids by sulfuric acid-methanol has been adapted to direct methylation of fatty acids of lipids in biological materials without prior extraction of the lipids. Successful application requires a solution or a suspension of fine particles of the lipid bearing material in ether. Concentrated sulfuric acid is added to the solution or suspension at low temperatures followed by addition of absolute methanol. The acid is neutralized by methanolic KOH and the esters extracted. Application of the method to prepare methyl esters of lipids in cream, blood serum, swine liver and kidney tissue, and cells of yeast onStaphylococcus aureus show that fatty acid composition based on this method compares with that determined by methylation of extracted lipids.

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Author notes

  1. Gertrude W. McGinnis

    Present address: , 2729 North Lake Boulevard, North St. Paul, Minnesota

  2. D. V. Vadehra

    Present address: Department of Food Science, Rice Hall, Cornell University, Ithaca, New York

Authors and Affiliations

  1. Department of Food Science, Michigan State University, East Lansing, Michigan

    L. R. Dugan Jr., Gertrude W. McGinnis & D. V. Vadehra

Authors
  1. L. R. Dugan Jr.
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  2. Gertrude W. McGinnis
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  3. D. V. Vadehra
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Additional information

Michigan Agricultural Experiment Station Publication No. 3803.

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Dugan, L.R., McGinnis, G.W. & Vadehra, D.V. Low temperature direct methylation of lipids in biological materials. Lipids 1, 305–308 (1966). https://doi.org/10.1007/BF02532671

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  • DOI: https://doi.org/10.1007/BF02532671

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