Abstract
Chloroform-methanol mixture was shown to extract acyl and alkyl dihydroxyacetone phosphate from enzyme incubation mixtures. However, when the lipid extract was washed with water to remove nonlipid materials, 70–80% of acyl and alkyl dihydroxyacetone phosphate were lost in the aqueous phase. It was shown that, keeping the pH low (<2.5) during the partition of lipids by the Bligh and Dyer method, most (>95%) of the acyl and alkyl dihydroxyacetone phosphate were recovered in the chloroform-rich phase. n-Butanol was shown to extract 80–90% of these lipids from incubation mixtures at neutral pH.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Hajra, A.K., in “Tumor Lipids: Biochemistry and Metabolism,” Edited by R. Wood, American Oil Chemists Society, Champaign, Ill., 1973, p. 183.
Snyder, F., Advan. Lipid Res. 10:233 (1972).
Snyder, F., R.L. Wykle, and B. Malone, Biochem. Biophys. Res. Commun. 34:315 (1969).
Kapoulas, V.M., and G.A. Thompson, Jr., Biochim. Biophys. Acta 187:594 (1969).
Friedberg, S.J., and A. Heifetz, Biochemistry 12:1100 (1973).
Bligh, E.G., and W.J. Dyer, Can. J. Biochem. 37:911 (1959).
Folch-Pi, J., M. Lees, and G.H. Sloane Stanley, J. Biol. Chem. 226:497 (1957).
Hajra, A.K., E.B. Seguin, and B.W. Arganoff, Ibid. 243:1609 (1968).
LaBelle, E.F., Jr., and A.K. Hajra, Ibid. 247:5825 (1972).
Hajra, A.K., Ibid. 243:3458 (1968).
Daae, L.N.W., and J. Bremer, Biochim. Biophys. Acta 270:23 (1972).
Hajra, A.K., and B.W. Agranoff, J. Biol. Chem. 243:1617 (1968).
Folch-Pi, J., and F.N. LeBaron, Fed. Proc. 12:203 (1953).
Long, C., R. Odavid, and E.J. Sargent, Biochem. J. 102:221 (1967).
Monroy, G., C.H. Rola, and M.E. Pullman, J. Biol. Chem. 247:6884 (1970).
Snyder, F., B. Malone, and M.C. Blank, Ibid. 247:1790 (1970).
Wykle, R.L., C. Piantadosi, and F. Snyder, Ibid. 247:2944 (1972).
Snyder, F., M. Clark, and C. Piantadosi, Biochem. Biophys. Res. Commun. 53:350 (1973).
Palmer St. C., F.B., Biochim. Biophys. Acta 231:134 (1971).
Daae, L.N.W., Ibid. 306:186 (1973).
Author information
Authors and Affiliations
About this article
Cite this article
Hajra, A.K. On extraction of acyl and alkyl dihydroxyacetone phosphate from incubation mixtures. Lipids 9, 502–505 (1974). https://doi.org/10.1007/BF02532495
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02532495