Abstract
Groups of rats were fed a choline-deficient (CD) or a choline-supplemented (CS) diet for 15 hr. Labeling of liver and plasma cephalins and lecithins was followed with time after injection of14C-L-methionine-methyl or 1–2-14C-2-dimethylaminoethanol, either alone or together with3H-S-adenosyl-L-methionine-methyl. A reduced concentration of liver and plasma lecithins was found in CD rats. In the same animals, labeling of the phospholipid fractions was considerably greater and faster than in CS rats. Administration of choline to rats previously fed the CD diet resulted in both an increased concentration of liver and plasma lecithins and a reduction in the labeling of liver and plasma lecithins to levels seen in control rats. These results suggest that in CD rats, while the overall synthesis of lecithins may be reduced due to insufficient availability of choline, the synthesis of lecithins via stepwise methylation of cephalins may be increased.
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Lombardi, B., Pani, P., Schlunk, F.F. et al. Labeling of liver and plasma lecithins after injection of 1–2-14C-2-dimethylaminoethanol and14C-L-methionine-methyl to choline deficient rats. Lipids 4, 67–75 (1969). https://doi.org/10.1007/BF02531797
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DOI: https://doi.org/10.1007/BF02531797