Skip to main content
SpringerLink
Log in

The effect of the environmental temperature on the fatty acid composition and on thein vivo incorporation of 1-14C-acetate in goldfish (Carassius auratus L.)

  • Published:
Lipids

Abstract

Two-year-old goldfish were adapted to 10C and 35C environmental temperatures during a three-week period, and the fatty acids from triglycerides and certain phospholipids were analyzed by gas-liquid chromatography. Over-all unsaturation of the major fatty acids increased with lower temperature in all lipids which were examined although fish maintained at 10C actually had less polyenoic acid in their tissues than did those maintained at 35C.

Fish acclimated to 10C and 30C were injected with 1-14C-acetate, and the activities of the isolated fatty acids were counted. The incorporation of14C into the fatty acids was much greater at the lower temperature. A comparison of the activities of saturated and unsaturated fatty acids within each temperature group revealed a tendency toward higher incorporation into the unsaturated acids at lower temperature. The possible correlations between accelerated biosynthesis of polyenoic acids and the lower tissue levels of these acids in the cold-adapted fish are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Henriques, V., and C. Hansen, Scand. Arch. Physiol.11, 151–155 (1901).

    Google Scholar 

  2. Fisher, H., K.G. Hollands, and H.S. Weiss, Proc. Soc. Exp. Biol. Med.110, 832–833 (1962).

    PubMed  CAS  Google Scholar 

  3. Fraenkel, G., and H.S. Hopf, Biochem. J.34, 1085–1092 (1940).

    PubMed  CAS  Google Scholar 

  4. Prill, E.A., P.R. Wench, and W.H. Peterson, Ibid.29, 21–33 (1935).

    PubMed  CAS  Google Scholar 

  5. Singh, J., and T.K. Walker, Res. Bull. Punjab Univ.92, 135–138 (1956).

    Google Scholar 

  6. Long, S.K., and O.B. Williams, J. Bacteriol.79, 629–637 (1960).

    PubMed  CAS  Google Scholar 

  7. Gaughran, E.R.C., Ibid.53, 506–507 (1947).

    Google Scholar 

  8. Pearson, L.K., and H.S. Raper, Biochem. J.21, 875–879 (1927).

    PubMed  CAS  Google Scholar 

  9. Marr, A. G., and J. L. Ingraham, J. Bacteriol.84, 1260–1267 (1962).

    PubMed  CAS  Google Scholar 

  10. Kates, M., and R. M. Baxter, Can. J. Biochem. Physiol.40, 1213–1227 (1962).

    PubMed  CAS  Google Scholar 

  11. Belehradek, J., Protoplasma12, 406–434 (1931).

    Article  Google Scholar 

  12. Holton, R. W., H. H. Blecker, and M. Onore, Phytochem.3, 595–602 (1964).

    Article  CAS  Google Scholar 

  13. Lewis, R. W., Comp. Biochem. Physiol.6, 75–89 (1962).

    Article  CAS  Google Scholar 

  14. Masoro, E. J., Physiol. Rev.46, 67–101 (1966).

    PubMed  CAS  Google Scholar 

  15. Brett, J. R., Quart. Rev. Biol.31, 75–87 (1956).

    Article  Google Scholar 

  16. Farkas, T., and S. Herodek, J. Lipid Res.5, 369–373 (1964).

    PubMed  CAS  Google Scholar 

  17. Kayama, M., Y. Tsuchiya, and J. F. Mead, Bull. Jap. Soc. Scient. Fish.29, 452–458 (1963).

    CAS  Google Scholar 

  18. Knipprath, W. G., and J. F. Mead, Fish. Ind. Res.3, 23–27 (1966).

    Google Scholar 

  19. Knipprath, W. G., and J. F. Mead, Lipids1, 113–117 (1966).

    Article  CAS  Google Scholar 

  20. Doudoroff, P., Biol. Bull. Wood's Hole83, 219–244 (1942).

    Google Scholar 

  21. Brett, J. R., Publ. Out. Fish.Res. Lab.63, 1–49 (1944).

    Google Scholar 

  22. O'Brien, J. S., D. L. Fillerup, and J. F. Mead, J. Lipid Res.5, 329–338 (1964).

    PubMed  Google Scholar 

  23. DeVries, B., JAOCS40, 184–186 (1963).

    CAS  Google Scholar 

  24. Goldfine, H., and K. Bloch, J. Biol. Chem.236, 2596–2601 (1961).

    PubMed  CAS  Google Scholar 

  25. Fry, F. E. J., J. R. Brett, and G. H. Clawson, Rev. Canad. Biol.1, 50–56 (1942).

    Google Scholar 

  26. Patkin, J. K., and E. J. Masoro, Am. J. Physiol.200, 847–850 (1961).

    PubMed  CAS  Google Scholar 

  27. Brenner, R. R., and R. O. Peluffo, J. Biol. Chem.241, 5213–5219 (1966).

    PubMed  CAS  Google Scholar 

  28. Barron, E. J., Biochem. Biophys. Acta116, 425–440 (1966).

    Article  PubMed  CAS  Google Scholar 

  29. Fulco, A. J., and J. F. Mead, J. Biol. Chem.235, 3379–3384 (1960).

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biophysics, Laboratory of Nuclear Medicine and Radiation Biology, UCLA School of Medicine, 90024, Los Angeles, California

    Werner G. Knipprath & James F. Mead

  2. Department of Biological Chemistry, UCLA School of Medicine, 90024, Los Angeles, California

    Werner G. Knipprath & James F. Mead

Authors
  1. Werner G. Knipprath
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. James F. Mead
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

These studies were supported in part by Contract AT(04-1) GEN-12 between the Atomic Energy Commission and the University of California.

Supported in part by PHS Research Career Award No. GM-K6-19, 177, from the Division of General Sciences, National Institutes of Health.

About this article

Cite this article

Knipprath, W.G., Mead, J.F. The effect of the environmental temperature on the fatty acid composition and on thein vivo incorporation of 1-14C-acetate in goldfish (Carassius auratus L.). Lipids 3, 121–128 (1968). https://doi.org/10.1007/BF02531728

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02531728

Keywords

Search

Navigation