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Isolation of cerebroside containing glucose (Glucosyl ceramide) and its possible significance in ganglioside synthesis

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Lipids

Abstract

A small amount of cerebroside containing glucose (glucosyl ceramide) was isolated from bovine brain by Florisil column chromatography and thin-layer chromatography. The fatty acids of the glucosyl ceramide were palmitic and stearic acids; small amounts of oleic and linoleic acids were present.

Rat brain tissue slices, incubated with U-14C-glucose, incorporated more radioacivity into glucosyl ceramide than into galactosyl ceramide. From these results the possible metabolic significance of the brain glucosyl ceramide in ganglioside metabolism is discussed.

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References

  1. Nishimura, K., N. Ueta, and T. Yamakawa, Japan. J. Exp. Med.36, 91–102 (1968).

    Google Scholar 

  2. Schwarz, H. P., L. Dreisbach, M. Barrionuevo, A. Kleschick and I. Kostyk, J. Lipid Res.2, 208–214 (1961).

    CAS  Google Scholar 

  3. Svennerholm, L., J. Neurochem.11, 839–853 (1964).

    Article  PubMed  CAS  Google Scholar 

  4. Pilz, H., K. Sandhoff and H. Jatzkewitz, Ibid.13, 1273–1282 (1968).

    Article  Google Scholar 

  5. Young, O. M., and J. N. Kanfer, J. Chromatogr.19, 611–613 (1965).

    Article  PubMed  CAS  Google Scholar 

  6. Kean, E. L., J. Lipid Res.,7, 449–452 (1968).

    Google Scholar 

  7. Folch, J., M. Lees and G. H. S. Stanley, J. Biol. Chem.226, 497–509 (1957).

    PubMed  CAS  Google Scholar 

  8. Radin, N. S., F. B. Lavin and J. R. Brown, Ibid.217, 789–796 (1955).

    PubMed  CAS  Google Scholar 

  9. Yokoyama, S., and T. Yamakawa, Japan. J. Exp. Med.34, 29–35 (1964).

    CAS  Google Scholar 

  10. Tamai, Y., T. Taketomi and T. Yamakawa, Ibid.37, 79–81 (1967).

    CAS  Google Scholar 

  11. Yamakawa, T., and N. Ueta, Ibid.34, 37–51 (1964).

    CAS  Google Scholar 

  12. Kochetkov, N. K., I. G. Zhukova and I. S. Glukhoded, Biokhimiya,29, 570–575 (1964).

    CAS  Google Scholar 

  13. Norton, W. T., and M. Brotz, Biochem. Biophys. Res. Comm.12, 198–203 (1963).

    Article  PubMed  CAS  Google Scholar 

  14. Radin, N. S., F. B. Martin and J. R. Brown, J. Biol. Chem.224, 499–507 (1957).

    PubMed  CAS  Google Scholar 

  15. Moser, H. W., and M. L. Karnovsky Ibid.234, 1990–1997 (1959).

    PubMed  CAS  Google Scholar 

  16. Burton, R. M., L. Garcia-Bunuel, M. Golden and Y. M. Balfour, Biochem.2, 580–585 (1963).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, Institute of Medical Science, Tokyo, Japan

    Kenji Nishimura

  2. Faculty of Medicine, University of Tokyo, Tokyo, Japan

    Tamio Yamakawa

Authors
  1. Kenji Nishimura
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  2. Tamio Yamakawa
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Nishimura, K., Yamakawa, T. Isolation of cerebroside containing glucose (Glucosyl ceramide) and its possible significance in ganglioside synthesis. Lipids 3, 262–266 (1968). https://doi.org/10.1007/BF02531199

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  • DOI: https://doi.org/10.1007/BF02531199

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