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Gas liquid chromatographic analysis of sphingosine bases in sphingolipids of human normal and multiple sclerosis cerebral white matter

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Lipids

Abstract

Sphingomyelins, cerebrosides and sulfatides were isolated from white matter of human normal and multiple sclerotic brain. Column chromatography was used for class separations, which were monitored by thin layer chromatography. Acid-catalyzed methanolysis, followed by isolation of bases and gas liquid chromatography of periodate-produced aldehydes, was used to determine sphingosine base composition of each sphingolipid. Reflux methanolysis (where HCl=2 N) was used for cerebrosides and sulfatides, but could not be used for reliable sphingomyelin base analysis, for which sealed-tube methanolysis at 70–80 C (where HCl=1 N) was used. Our results suggest there is little, if any, difference in sphingosine base chain-length distribution among the sphingolipids analyzed.

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Authors and Affiliations

  1. Biochemistry Department, The University of Texas Southwestern Medical School at Dallas, 75235, Texas

    Ezio A. Moscatelli & Elizabeth Isaacson

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  1. Ezio A. Moscatelli
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  2. Elizabeth Isaacson
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Moscatelli, E.A., Isaacson, E. Gas liquid chromatographic analysis of sphingosine bases in sphingolipids of human normal and multiple sclerosis cerebral white matter. Lipids 4, 550–555 (1969). https://doi.org/10.1007/BF02531040

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  • DOI: https://doi.org/10.1007/BF02531040

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