Abstract
The aim of this study was to develop a convenient method for the preparation and study of a soluble delipidated form of human serum β-lipoprotein. This was achieved by succinylation and delipidation with ether-ethanol (3∶1). The succinylated apoprotein was soluble in either 0.13 M Tris-HCl buffer, pH 8.2 (for β-lipoprotein prepared by ultracentrifugation) or in the same Tris buffer to which 5 mM sodium decyl sulfate was added (for heparin-Mn precipitated β-lipoprotein). The immunological activity of β-lipoprotein or its apoprotein were markedly altered by succinylation. Whereas the succinylated β-lipoprotein appeared as one peak in the analytical ultracentrifuge, the succinylated apoprotein appeared as two. Under the electron microscope β-lipoprotein and succinylated β-lipoprotein were indistinguishable, appearing as uniform preparations of spherical particles 215 to 220 A in diamater.
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Gotto, A.M., Levy, R.I. & Fredrickson, D.S. Preparation and properties of an apoprotein derivative of human serum β-lipoprotein. Lipids 3, 463–470 (1968). https://doi.org/10.1007/BF02530887
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DOI: https://doi.org/10.1007/BF02530887