Abstract
Triacylglycerol is accumulated byStreptomyces spp. when grown in submerged culture. Ultrastructural studies using transmission electron microscopy (TEM), staining and freeze-fracture/freeze-etch procedures, and light microscopy confirmed the accumulation of neutral lipid byS. lividans andS. coelicolor during the stationary phase and its storage within membrane-bound globular structures within the cytoplasm. There structures were of various sizes and occupied up to approximately 80% of the total cell volume at that time. There was no evidence of such material within cells examined during the early exponential phase of growth. The globules visualised by TEM were electron-transparent since they comprised lipids containing saturated fatty acids that did not react with osmium tetroxide. The globules appeared to be bounded by a single membrane.
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Abbreviations
- TAG :
-
triacylglycerol
- TEM :
-
transmission electron microscopy
References
Ballio A, Barcellona S, Boniforti L (1965) The component fatty acids of lipids from someStreptomyces species. Biochem J 94:11C-13C
Braña AF, Manzanal MB, Hardisson C (1982) Characterization of intracellular polysaccharides ofStreptomyces. Can J Microbiol 28:1320–1323
Braña AF, Méndez C, Díaz LA, Manzanal MB, Hardisson C (1986) Glycogen and trehalose accumulation during colony development inStreptomyces antibioticus. J Gen Microbiol 132: 1319–1326
Burdon KL (1946) Fatty material in bacteria and fungi revealed by staining dried, fixed slide preparations. J Bacteriol 52:665–678
Chater KF, Merrick MJ (1979) Streptomycetes. In: Parish JH (ed) Developmental biology of prokaryotes. University of California Press, Berkeley Los Angeles, pp 93–114
Dawes (1985) Starvation, survival and energy reserves. In: Fletcher M, Floodgate GD (eds) Bacteria in their natural environments. Society of General Microbiology Special Publication, no 16. Academic Press, London, pp 43–79
Daza A, Martin JF, Dominguez A, Gil JA (1989) Sporulation of several species ofStreptomyces in submerged cultures after nutritional downshift, J Gen Microbiol 235:2483–2491
Deetz JS, Behrman EJ (1981) Reaction of osmium reagents with amino acids and proteins. Int J Pept Protein Res 17:495–500
Friend DS (1969) Cytochemical staining of multivesicular body and Golgi vesicles. J Cell Biol 41:269–279
Gale GR, McLlain HH (1963) Effect of ethambutol on cytology ofMycobacterium smegmatis. J Bacteriol 86:749–756
Gurr MI (1980) The biosynthesis of triacylglycerols. In: Stumpf PK (ed) The biochemistry of plants, vol 4. Academic Press, New York London, pp 205–249
Gurr MI, Harwood JL (1991) Lipids as energy stores. In: Lipid biochemistry, 4th edn. Chapman & Hall, London, pp 126–130
Gurr MI, Blades J, Appleby RS, Smith CG, Robinson MP, Nichols BW (1974) Triglyceride biosynthesis and storage in whole seeds and oil bodies ofCrambe abyssinica. Eur J Biochem 43:281–290
Hayat MA (1989) Chemical fixation. In: Principles and techniques of electron microscopy: biological applications, 3rd edn. Macmillan, London, pp 42–46
Holdsworth JE, Veenhuis M, Ratledge C (1988) Enzyme activities in oleaginous yeasts accumulating and utilizing exogenous or endogenous lipids. J Gen Microbiol 134:2907–2915
Hopwood DA (1967) Genetic analysis and genome structure inStreptomyces coelicolor. Bacteriol Rev 31:373–403
Jensen TE, Sicko LM (1971) Fine structure of poly-β-hydroxybutyric acid granules in a blue-green alga,Chlorogloea fritschii. J Bacteriol 106:683–686.
Kannan LV, Rehacek Z (1970) Formation of poly-β-hydroxybutyrate by Actinomycetes. Indian J Biochem 7:126–129
Martin JF, Demain AL (1980) Control of antibiotic synthesis. Microbiol Rev 44:230–251
Olukoshi ER, Packter NM (1994) Importance of stored triacylglycerols inStreptomyces: possible carbon source for antibiotics. Microbiology 140:931–943
Packter NM, Flatman S, Lucock AJ (1985) Formation of storage lipids and actinorhodin, a phenolic antibiotic inStreptomyces coelicolor. Biochem Soc Trans 13:251–252
Ranade N, Vining LC (1993) Accumulation of intracellular carbon reserves in relation to chloramphenicol biosynthesis byStreptomyces venezuelae. Can J Microbiol 39:377–383
Reynolds ES (1963) The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J Cell Biol 17:208–212
Ryter A, Kellenberger E (1958) Étude au microscope électronique de plasmas contenant de l'acide désoxyribonucleique. Z Naturforsch 13B:597–605
Shively JM (1974) Annu Rev Microbiol 28:167–187
Wildermuth H, Hopwood DA (1970) Septation during sporulation inStreptomyces coelicolor. J Gen Microbiol 60:51–59
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Packter, N.M., Olukoshi, E.R. Ultrastructural studies of neutral lipid localisation inStreptomyces . Arch. Microbiol. 164, 420–427 (1995). https://doi.org/10.1007/BF02529740
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DOI: https://doi.org/10.1007/BF02529740