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Sterol composition ofPhaeodactylum tricornutum as influenced by growth temperature and light spectral quality

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Lipids

Abstract

In a detailed sterol analysis of the marine diatomPhaeodactylum tricornutum, free sterols as well as esterified and glycosylated conjugates were found. When the alga was grown under standard conditions (i.e., at 13°C under white light), 64% of total sterols were steryl glycosides. In all sterol classes, except steryl esters, (24S)-24-methylcholesta-5,22E--dien-3β-ol (epibrassicasterol) was the major (80 to 99%) sterol component. Eight other sterols were identified. Growth under different light spectral quality (red, blue, yellow, and green) at 13 and 23°C was examined. At 23°C, a dramatic decrease in total sterol content was observed, especially under blue light. The distribution of sterols between free and conjugated forms as well as sterol profile inside each class was found to be strongly dependent on the light spectral quality at both temperatures.

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Abbreviations

ASG:

acylated steryl glycosides

FS:

free sterols

GC:

gas chromatography

MS:

mass spectrometry

SE:

steryl esters

SG:

steryl glycosides

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Authors and Affiliations

  1. Laboratoire de Biologie et Biotechnologies Marines, Université de Caen Basse-Normandie, 14032, Caen, France

    Benoît Véron, Chantal Billard & Jean-Claude Dauguet

  2. Institut de Biologie Moléculaire des Plantes, UPR CNRS 406, 67083, Strasbourg, France

    Marie-Andrée Hartmann

Authors
  1. Benoît Véron
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  2. Chantal Billard
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  3. Jean-Claude Dauguet
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  4. Marie-Andrée Hartmann
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Véron, B., Billard, C., Dauguet, JC. et al. Sterol composition ofPhaeodactylum tricornutum as influenced by growth temperature and light spectral quality. Lipids 31, 989–994 (1996). https://doi.org/10.1007/BF02522694

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  • DOI: https://doi.org/10.1007/BF02522694

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