Abstract
Atmospheric pressure chemical ionization-mass spectrometry (APCI-MS) was used for quantitative analysis of triglycerides (TG) separated by reverse-phase high-performance liquid chromatography. APCI-MS was used for analysis of mono-acid TG standards containing deuterated internal standard, of a synthetic mixture of heterogeneous TG, of randomized and normal soybean oils and of randomized and normal lard samples. Quantitation of the TG by four approaches based on APCI-MS were compared, and these were compared to quantitation obtained using liquid chromatography (LC) with flameionization detection (FID). The APCI-MS methods were based on (i) calibration curves from data for mono-acid TG standards, (ii) response factors obtained from a synthetic mixture of TG, (iii) response factors calculated from comparison of randomized samples to their statistically expected compositions, and (iv) response factors calculated from comparison of fatty acid (FA) compositions calculated from TG compositions to FA compositions obtained by calibrated gas chromatography (GC) with FID. Response factors derived from a synthetic mixture were not widely applicable to samples of disparate composition. The TG compositions obtained using APCI-MS data without application of response factors had average relative errors very similar to those obtained using LC-FID. Numerous TG species were identified using LC/APCI-MS which were undetected using LC-FID. Two quantitation methods, based on response factors calculated from randomized samples or on response factors calculated from FA compositions, both gave similar results for all samples. The TG compositions obtained using response factors calculated from FA compositions showed less average relative error than was obtained from LC-FID data, and were in good agreement with predicted compositions for the synthetic mixture and for randomized soybean oil and lard samples.
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Abbreviations
- ACN:
-
acetonitrile
- APCI-MS:
-
atmospheric pressure chemical ionization-mass spectrometry
- DG:
-
diglyceride
- EIC:
-
extracted ion chromatogram
- ELSD:
-
evaporative light-scattering detector
- FA:
-
fatty acid
- FAME:
-
fatty acid methyl esters
- FID:
-
flame-ionization detector
- GC:
-
gas chromatography
- L:
-
linoleic acid (18∶2)
- LC:
-
liquid chromatography
- Ln:
-
linolenic acid (18∶3)
- M:
-
myristic acid (14∶0)
- O:
-
oleic acid (18∶1)
- P:
-
palmitic acid (16∶0)
- Po:
-
palmitoleic acid (16∶1)
- RP-HPLC:
-
reverse-phase high-performance liquid chromatography
- S:
-
stearic acid (18∶0)
- SBO:
-
soybean oil
- TG:
-
triglyceride
References
Shukla, V.K.S. (1988) Recent Advances in the High-Performance Liquid Chromatography of Lipids,Prog. Lipid Res., 27, 5–38.
Ruiz-Gutierrez, V., and Barron, L.J.R. (1995) Methods for the Analysis of Triacylglycerols,J. Chromatogr. B 671, 133–168.
Myher, J.J., and Kuksis, A. (1995) General Strategies in Chromatographic Analysis of Lipids,J. Chromatogr. B 671, 3–33.
Byrdwell, W.C., and Emken, E.A. (1995) Analysis of Triglycerides Using Atmospheric Pressure Chemical Ionization Mass Spectrometry,Lipids 30, 173–175.
Neff, W.E., and Byrdwell, W.C. (1995) Soybean Oil Triacylglycerol Analysis by Reverse-Phase High-Performance Liquid Chromatography Coupled with Atmospheric Pressure Chemical Ionization Mass Spectrometry,J. Am. Oil Chem. Soc. 72, 1185–1191.
Adlof, R.O., Miller, W.R., and Emken, E.A. (1978) Synthesis ofcis andtrans Methyl 8- and 13-Octadecenoate-d 2 andd 4 Isomers.J. Label. Comp. Radiopharm. 15, 625.
Osborn, J.A., Jardine, F.H., Young, J.F., and Wilkinson, G. (1966) The Preparation and Properties ofTris(triphenylphosphine)halo-genorhodium(1) and Some Reactions Thereof Including Catalytic Homogeneous Hydrogenation of Olefins and Acetylenes and Their Derivatives,J. Chem. Soc. (A), 1711–1732.
Wheeler, D.H., Reimenschneider, R.W., and Sando, C.E. (1940) Preparation, Properties, and Thiocyanogen Absorbtion of Triolein and Trilinolein,J. Biol. Chem. 132, 687–699.
List, G.R., Emken, E.A., Kwolek, W.F., and Simpson, T.D. (1977), “Zerotrans” Margarines: Preparation, Structure, and Properties of Interesterified Soybean Oil-Soy Trisaturate Blends,J. Am. Oil Chem. Soc. 54, 408–413.
Glass, R.L. (1969) Preparation of Milk Fat Methyl Esters by Alcoholysis in an Essentially Nonalcoholic Solution,J. Dairy Sci. 52, 1289–1290.
Zeitoun, M.A.M., Neff, W.E., Selke, E., and Mounts, T.L. (1991) Analyses of Vegetable Oil Triglyceride Molecular Species by Reverse-Phase High-Performance Liquid Chromatography,J. Liquid Chromatogr. 14, 2685–2698.
Neff, W.E., Zeitoun, M.A.M., and Weisleder, D. (1992) Resolution of Lipolysis Mixtures from Soybean Oil by a Solid-Phase Extraction Procedure,J. Chromatogr. 589, 353–357.
Daubert, B.F. (1949) The Composition of Fats,J. Am. Oil. Chem. Soc. 26, 556–558.
Bailey, A.E., and Swern, D. (1964)Bailey's Industrial Oil and Fat Products, 3rd edn., pp. 965–968, John Wiley & Sons, New York.
Mares, P., Rezanka, T., and Novak, M. (1991) Analysis of Human Blood Plasma Triacylglycerols Using Capillary Gas Chromatography, Silver Ion Thin-Layer Chromatographic Fractionation and Desorption Chemical Ionization Mass Spectrometry,J. Chromatogr., 568, 1–10.
Rezanka, T., and Mares, P. (1991) Determination of Plant Triacylglycerols Using Capillary Gas Chromatography, High-Performance Liquid Chromatography and Mass Spectrometry,J. Chromatogr. 542, 145–159.
Myher, J.J., Kuksis, A., Marai, L., and Manganaro, F. (1984) Quantitation of Natural Triacylglycerols by Reverse-Phase Liquid Chromatography with Direct Liquid Inlet Mass Spectrometry,J. Chromatogr. 283, 289–301.
Kuksis, A., Marai, L., and Myher, J.J. (1983) Strategy of Glycerolipid Separation and Quantitation by Complementary Analytical Techniques,J. Chromatogr. 273, 43–66.
Hites, R.A. (1970) Quantitative Analysis of Triglyceride Mixtures by Mass Spectrometry,Anal. Chem. 42, 1736–1740.
Phillips, F.C., Erdahl, W.L., Schmit, J.A., and Privett, O.S. (1984) Quantitative Analysis of Triglyceride Species of Vegetable Oils by High Performance Liquid Chromatographyvia a Flame-Ionization Detector,Lipids 19, 880–887.
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Byrdwell, W.C., Emken, E.A., Neff, W.E. et al. Quantitative analysis of triglycerides using atmospheric pressure chemical ionization-mass spectrometry. Lipids 31, 919–935 (1996). https://doi.org/10.1007/BF02522685
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DOI: https://doi.org/10.1007/BF02522685