Skip to main content
SpringerLink
Log in

Soluble CD4 and CD8 in serum from patients with localized scleroderma

  • Original Paper
  • Published:
Archives of Dermatological Research Aims and scope Submit manuscript

Abstract

Localized scleroderma has been shown to be accompanied by various immunologic abnormalities. To obtain functional information on activated CD4+ or CD8+ T cells, we studied the levels of soluble CD4 (sCD4) and soluble CD8 (sCD8) in serum from patients with localized scleroderma. Serum samples were examined by enzyme-linked immunosorbent assay. The samples were obtained from 49 patients in the following three subgroups: 15 patients with generalized morphea, 22 with linear scleroderma, and 12 with morphea. The levels of sCD4 and sCD8 were significantly elevated in patients with generalized morphea. Furthermore, these patients showed significantly higher levels of sCD4 than those with systemic sclerosis (SSc). The frequency of positivity for IgG anti-single-stranded DNA (ssDNA) antibody was significantly higher in localized scleroderma patients with elevated sCD4 levels than in patients with normal sCD4 levels. The frequency of positivity for antinuclear antibodies, IgM antihistone antibodies, IgG anti-ssDNA antibody and rheumatoid factor, and elevated sCD23 levels were significantly higher in localized scleroderma patients with elevated sCD8 levels than in patients with normal sCD8 levels. Our findings suggest that both CD4+ and CD8+ T cells are activated in vivo in generalized morphea and that the immunologic events in generalized morphea are different from those in SSc.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Takehara K, Moroi Y, Nakabayashi Y, Ishibashi Y (1983) Antinuclear antibodies in localized scleroderma. Arthritis Rheum 26:612–616

    PubMed  CAS  Google Scholar 

  2. Ruffatti A, Peserico A, Glorioso S, Fiocco U, Rossi L, Gambari P, Todesco S (1986) Anticentromere antibody in localized scleroderma. J Am Acad Dermatol 15:637–642

    PubMed  CAS  Google Scholar 

  3. Falanga V, Medsger TA Jr, Reichlin M, Rodnan GP (1986) Linear scleroderma: clinical spectrum, prognosis, and laboratory abnormalities. Ann Intern Med 104:849–857

    PubMed  CAS  Google Scholar 

  4. Falanga V, Medsger TA Jr, Reichlin M (1987) Antinuclear and anti-single-stranded DNA antibodies in morphea and generalized morphea. Arch Dermatol 123:350–353

    Article  PubMed  CAS  Google Scholar 

  5. Kikuchi K, Takehara K, Ishibashi Y (1989) Antinuclear antibodies in localized scleroderma: unique staining in chromosome spreads. J Am Acad Dermatol 21:1301–1303

    Article  PubMed  CAS  Google Scholar 

  6. Sato S, Ihn H, Soma Y, Igarashi A, Tamaki T, Kikuchi K, Ishibashi Y, Takehara K (1993) Antihistone antibodies in patients with localized scleroderma. Arthritis Rheum 36:1137–1141

    PubMed  CAS  Google Scholar 

  7. Scarola JA, Shulman LE (1975) Serologic abnormalities and their significance in localized scleroderma (abstract). Arthritis Rheum 18:526

    PubMed  CAS  Google Scholar 

  8. Falanga V, Medsger TA Jr, Reichlin M (1985) High titers of antibodies to single-stranded DNA in linear scleroderma. Arch Dermatol 121:345–347

    Article  PubMed  CAS  Google Scholar 

  9. Takehara K, Kikuchi K, Soma Y, Igarashi A, Ishibashi Y (1990) Anti-single-stranded DNA antibody and muscle involvement in localized scleroderma. Arch Dermatol 126:1368–1369

    Article  PubMed  CAS  Google Scholar 

  10. Sato S, Fujimoto M, Ihn H, Kikuchi K, Takehara K (1994) Antigen specificity of antihistone antibodies in localized scleroderma. Arch Dermatol 130:1273–1277

    Article  PubMed  CAS  Google Scholar 

  11. Sato S, Fujimoto M, Kikuchi K, Ihn H, Takehara K (1996) Elevated soluble CD23 levels in the sera from patients with localized scleroderma. Arch Dermatol Res 288 (in press)

  12. Ihn H, Sato S, Fujimoto M, Kikuchi K, Takehara K (1995) Demonstration of interleukin-2, interleukin-4 and interleukin-6 in sera from patients with localized scleroderma. Arch Dermatol Res 287:193–197

    Article  PubMed  CAS  Google Scholar 

  13. Uziel Y, Krafchik BR, Feldman B, Silverman ED, Rubin LA, Laxer RM (1994) Serum levels of soluble interleukin-2 receptor: a marker of disease activity in localized scleroderma. Arthritis Rheum 37:898–901

    PubMed  CAS  Google Scholar 

  14. Sato S, Fujimoto M, Ihn H, Kikuchi K, Takehara K (1994) Clinical characteristics associated with antihistone antibodies in patients with localized scleroderma. J Am Acad Dermatol 31:1273–1277

    Google Scholar 

  15. Subcommittee for Scleroderma Criteria of the American Rheumatism Association Diagnostic and Therapeutic Criteria Committee (1986) Preliminary criteria for the classification of systemic sclerosis (scleroderma). Arthritis Rheum 26:612–616

    Google Scholar 

  16. LeRoy EC, Krieg T, Black C, Medsger TA Jr, Fleischmajer R, Rowell N, Jablonska, S, Wollheim F (1988) Scleroderma (systemic sclerosis): classification, subsets and pathogenesis. J Rheumatol 15:202–205

    PubMed  CAS  Google Scholar 

  17. Sato S, Ihn H, Kikuchi K, Takehara K (1994) Antihistone antibodies in systemic sclerosis: association with pulmonary fibrosis. Arthritis Rheum 37:391–394

    PubMed  CAS  Google Scholar 

  18. Fujimoto J, Levy S, Levy R (1983) Spontaneous release of the Leu-2 (T8) molecule from human T cells. J Exp Med 159:752–756

    Article  Google Scholar 

  19. Fujimoto J, Stewart S, Levy R (1984) Immunochemical analysis of the released Leu-2 (T8) molecules. J Exp Med 160:116–124

    Article  PubMed  CAS  Google Scholar 

  20. Giblin P, Ledbetter JA, Kavathas P (1989) A secreted form of the human lymphocyte cell surface molecule CD8 arises from alternative splicing. Proc Natl Acad Sci USA 86:998–1002

    Article  PubMed  CAS  Google Scholar 

  21. Tomkinson BE, Brown MC, Ip SH, Carrabis S, Sullivan JL (1989) Soluble CD8 during T cell activation. J Immunol 142:2230–2236

    PubMed  CAS  Google Scholar 

  22. Sawada S, Sugai S, Iijima S, Takei M, Paredes E, Hayama T, Nishinavita S, Hosokawa Y, Horie T, Obara T (1992) Increased soluble CD4 and decreased soluble CD8 molecules in patients with Sjøgren's syndrome. Am J Med 92:134–140

    Article  PubMed  CAS  Google Scholar 

  23. Furukawa S, Matsubara T, Tsuji K, Motohashi T, Okumura K, Yabata K (1991) Serum soluble CD4 and CD8 levels in Kawasaki disease. Clin Exp Immunol 86:134–139

    Article  PubMed  CAS  Google Scholar 

  24. Lipnick RN, Sfikakis PP, Klipple GL, Tsokos GC (1993) Elevated soluble CD8 antigen and soluble interleukin-2 receptors in the sera of patients with juvenile rheumatoid arthritis. Clin Immunol Immunopathol 68:64–67

    Article  PubMed  CAS  Google Scholar 

  25. Symons JA, Wood NC, Di Giovine FS, Duff GW (1990) Soluble CD8 in patients with rheumatic diseases. Clin Exp Immunol 80:354–359

    Article  PubMed  CAS  Google Scholar 

  26. Famularo G, Giacomelli R, Sacchetti S, Danese C, Luciani AM, Perego MA, Tonietti G (1990) Soluble CD8 antigen in systemic sclerosis. J Clin Lab Immunol 32:109–112

    PubMed  CAS  Google Scholar 

  27. Tokano Y, Kaneko H, Obara T, Hashimoto H, Okumura K, Hirose S (1993) Soluble CD4 and CD8 molecules in patients with systemic sclerosis. Clin Rheumatol 12:490–493

    Article  PubMed  CAS  Google Scholar 

  28. Kahaleh MB (1991) Soluble immunologic products in scleroderma sera. Clin Immunol Immunopathol 58:139–144

    Article  PubMed  CAS  Google Scholar 

  29. Reinherz EL, Kung PC, Goldstein G, Schlossman SF (1979) Separation of functional subsets of human T cells by a monoclonal antibody. Proc Natl Acad Sci USA 76:4061–4065

    Article  PubMed  CAS  Google Scholar 

  30. Deen KC, McDougal JS, Inacker R, Folena-Wasserman G, Arthos J, Rosenberg J, Maddon PJ, Axel R, Sweet RW (1988) A soluble form of CD4 (T4) protein inhibits AIDS virus infection. Nature 331:82–84

    Article  PubMed  CAS  Google Scholar 

  31. Kurane I, Innis BL, Nimmannitya S, Nisalak A, Meager A, Janis J, Vergani D (1991) Activation of T lymphocytes in Dengue virus infections. J Clin Invest 88:1473–1480

    Article  PubMed  CAS  Google Scholar 

  32. Peakman M, Senaldi G, Foote N, McManus TJ, Vergani D (1992) Naturally occurring soluble CD4 in patients with human immunodeficiency virus infection. J Infect Dis 165:799–804

    PubMed  CAS  Google Scholar 

  33. Symons JA, McCulloch JF, Wood NC, Duff GW (1991) Soluble CD4 in patients with rheumatoid arthritis and osteoarthritis. Clin Immunol Immunopathol 60:72–82

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Dermatology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo Bunkyo-ku, 113, Tokyo, Japan

    Shinichi Sato, Manabu Fujimoto, Hironobu Ihn & Kunihiko Tamaki

  2. Department of Dermatology, University of Tokyo Branch Hospital, 3-28-6 Mejirodai, Bunkyo-ku, 112, Tokyo, Japan

    Kanako Kikuchi

  3. Department of Dermatology, Kanazawa University School of Medicine, Kanazawa, Japan

    Kazuhiko Takehara

Authors
  1. Shinichi Sato
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Manabu Fujimoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kanako Kikuchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hironobu Ihn
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Kunihiko Tamaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Kazuhiko Takehara
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sato, S., Fujimoto, M., Kikuchi, K. et al. Soluble CD4 and CD8 in serum from patients with localized scleroderma. Arch Dermatol Res 288, 358–362 (1996). https://doi.org/10.1007/BF02507103

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02507103

Key words

Search

Navigation