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Culture of cells derived from the human sebaceous gland under serum-free conditions without a biological feeder layer or specific matrices

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Abstract

We succeeded in serially culturing cells derived from human sebaceous gland (sebocytes) under serum-free conditions. Sebaceous glands were isolated from dispase-treated facial skin specimens and cultured using two different methods, explant culture and dispersed cell culture, in KGM. In both types of culture the sebocytes proliferated rapidly without a biological feeder layer or specific matrices. It was possible to cultivate the cells serially for at least three passages (explant culture) and six passages (dispersed cell culture), and to store the cells in liquid nitrogen with good recovery. Analytical thin-layer chromatography revealed that the cells synthesized a large amount of sebum-specific lipids, squalene and wax esters in vitro. Both testosterone and 5α-dihydrotestosterone significantly stimulated the proliferation of the sebocytes. The methods presented here may permit a large series of studies on the function of sebaceous glands or cells derived from sebaceous glands.

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Authors and Affiliations

  1. Department of Dermatology, School of Medicine, The University of Tokushima, Kuramoto-cho 3, 770, Tokushima, Japan

    Takeshi Fujie, Takanori Shikiji, Naoyuki Uchida, Yoshio Urano, Hiroaki Nagae & Seiji Arase

Authors
  1. Takeshi Fujie
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  2. Takanori Shikiji
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  3. Naoyuki Uchida
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  4. Yoshio Urano
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  5. Hiroaki Nagae
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  6. Seiji Arase
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Fujie, T., Shikiji, T., Uchida, N. et al. Culture of cells derived from the human sebaceous gland under serum-free conditions without a biological feeder layer or specific matrices. Arch Dermatol Res 288, 703–708 (1996). https://doi.org/10.1007/BF02505281

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  • DOI: https://doi.org/10.1007/BF02505281

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