Summary
The retention behaviour of highly polar and charged disaccharide isomers has been studied on porous graphitic carbon columns and experimental parameters were varied over wide a range, including concentration and type of mobile phase constituents as well as temperature. The hydrophilic and anionic sugar analytes were highly retained on this stationary phase compared to the octadecyl-derivatized silica packings more commonly used. E.g., an increase in retention with polarity of a solute and with temperature was observed. By isotherm measurements and nonlinear fitting of Langmuirian expressions to the experimental data the graphite surface appeared homogeneous with only one kind of active adsorption site for these kinds of compound which was furthermore supported by the linear Van't Hoff plots obtained by varying the temperature. The gain in free energy was found to be entropically driven after determination of the ΔH° and ΔS° values. However, enthalpy-entropy compensation behavior was not met.
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Hennion, M.-C.; Coquart, V.; Guenu, S.; Sella, C.J. Chromatogr. A 1995,712, 287–301.
Forgács, E.; Cserháti, T.J. Chromatogr. 1992,600, 43–49.
Möckel, H.J.; Braedikow, A.; Melzer, H.; Aced, G.J. Liq. Chromatogr. 1991,14(13), 2477–2498.
Tanaka, N.; Tanigawa, T.; Kimata, K.; Hosoya, K.; Araki, T.J. Chromatogr. 1991,549, 29–41.
Bassler, B.J.; Kaliszan, T.; Hartwick, R.A.J. Chromatogr. 1989,461, 139–147.
Lim, C.K.Biomed. Chromatogr. 1989,3, 92–93.
Elfakir, C.; Dreux, M.J. Chromatogr. 1996,727, 71–82.
Elfakir, C.; Chaimbault, P.; Dreux, M.J. Chromatogr. A 1998,829, 193–199.
Mercier, J.-P.; Morin, P.; Dreux, M.; Tambuté, A.J. Chromatogr. A 1999,849, 197–207.
Stefansson, M.; Lu, B.Chromatographia 1993,35, 61–66.
Knox, J.H.; Ross, P.Advances in Chromatography; Marcel Dekker Inc.: New York, 1997; Vol. 37, pp 73–119.
Ross, P.LC-GC 2000,13(5), 310–319.
Svensson, L.; Markides, K.E.J. Microcol. Sep. 1994,6, 409–414.
Davies, M.J.; Hounsell, E.F.J. Chromatogr. A 1996,720, 227–233.
Mada, A.; Toyoda, H.; Imanari, T.Anal. Sci. 1992,8, 793–797.
Stefansson, M.; Hoffman, K.-J.Chirality 1992,4, 509–514.
Snyder, R.L.Principles of Adsorption Chromatography, Dekker: New York, 1968.
Forgács, E.; Cserháti, T.; Valkó, K.J. Chromatogr. 1992,592 75–83.
Cserháti, T.; Forgács, E.J. Chromatogr. 1993,643, 331–336.
Forgács, E.; Cserháti, T.J. Pharm. Biomed. Anal. 1998,18, 15–20.
Nèmeth-Kiss, V.; Forgács, E.; Cserháti, T.J. Chromatogr. A 1997,776, 147–152.
Stefansson, M.Unpublished Work.
Valkó, K.; Snyder, L.R.; Glajch, J.L.,J. Chromatogr. A 1993,656, 501–520.
The equation used for calculation is: 1/ε=0.0126+0.0173χ where χ is the mole fraction of acetonitrile.
Gu, G.; Lim, C.K.J. Chromatogr. 1990,515, 183–192.
Martin, A.J.P.Biochem. Soc. Symp. 1950,3, 4.
Dutton, G.J.Glucuronic Acid. Free and Combined; Academic Press: New York, 1966.
Guiochon, G.; Golshan-Shirazi, S.; Katti, A.M.Fundamentals of Preparative and Nonlinear Chromatography Academic Press Inc:1994; Chapter 3.
Koizumi, K.; Okada, Y.; Fukuda, M.Carbohydr. Res. 1991,215, 67–80.
Melander, W.; Campbell, D.E.; Horváth, C.J. Chromatogr. 1978,158, 215–225.
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Koivisto, P., Stefansson, M. Retention studies of sulphated glycosaminoglycan disaccharides on porous graphitic carbon capillary columns. Chromatographia 57, 37–45 (2003). https://doi.org/10.1007/BF02497475
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DOI: https://doi.org/10.1007/BF02497475