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Simultaneous determination of retinol acetate, retinol palmitate, cholecalciferol, α-tocopherol acetate and alphacalcidol in capsules by non-aqueous reversed-phase HPLC and column backflushing

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Summary

A very simple non-aqueous reversed-phase HPLC method has been developed for analysis of retinol acetate, retinol palmitate, cholecalciferol, α-tocopherol acetate and alphacalcidol in capsules without the need for saponification. A reversed-phase (LiChrospher C8, 4.6 mm i.d.) column is used with acetonitrile-methanol, 95∶5 (v/v) as mobile phase at flow rate of 1 mL min−1. Sample treatment consists only in dilution of the capsule contents withn-hexane and methanol. This method is suitable for routine quantification in the industrial quality-assurance laboratory.

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Authors and Affiliations

  1. Glaxo Wellcome Poznan S. A, ul. Grunwaldzka 189, 60-322, Poznan, Poland

    S. Wielinski

  2. Institute of Chemical Technology and Engineering, Poznan University of Technology, Pl. M. Sklodowskiej-Curie 2, 60-965, Poznan, Poland

    A. Olszanowski

Authors
  1. S. Wielinski
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  2. A. Olszanowski
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Wielinski, S., Olszanowski, A. Simultaneous determination of retinol acetate, retinol palmitate, cholecalciferol, α-tocopherol acetate and alphacalcidol in capsules by non-aqueous reversed-phase HPLC and column backflushing. Chromatographia 50, 109–112 (1999). https://doi.org/10.1007/BF02493627

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  • DOI: https://doi.org/10.1007/BF02493627

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