Abstract
By extracting the cell wall fraction obtained from hypocotyls of soybean seedlings with 1 M NaCl solution, a factor which caused the gelation of the pectin solution was released. Mixed with the extract, the pectin solution increased in viscosity and eventually solidified. The pectin-gelatinizing factor (PGF) was of protein nature and seemed to modify pectin molecules to make them coagulate. The physiological significance of the PGF action was discussed. PGF seemed to be widely distributed among seed plants and to be cell wall-boundin situ.
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References
Albersheim, P., H. Neukom andH. Deuel. 1960. Splitting of pectin chain molecules in neutral solutions. Arch. Biochem. Biophys.90: 46–51.
Barrett, A.J. andD.H. Northcote. 1965. Apple fruit pectic substances. Biochem. J.94: 617–627.
Dubois, M., K.A. Gilles, J.K. Hamilton, P.A. Rebers andF. Smith. 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem.28: 350–356.
Lowry, O.H., N.J. Rosebrough, A.L. Farr andR.J. Randall 1951. Protein measurement with Folin phenol reagent. J. Biol. Chem.193: 265–279.
Yamaoka, T. andS. Sato. 1977. Analysis of extracellular macromolecules of cultured tobacco cells in comparison with cell-wall macromolecules. Bot. Mag. Tokyo90: 153–163.
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Yamaoka, T., Sato, F. A study on a cell wall-bound factor which causes the gelation of pectin solution. Bot Mag Tokyo 94, 335–341 (1981). https://doi.org/10.1007/BF02493394
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DOI: https://doi.org/10.1007/BF02493394