Summary
A bioanalytical method using solid-phase extraction and high-performance liquid chromatography (LC) has been developed and validated for the determination of amodiaquine, chloroquine and their metabolites in 100 μL blood applied to sampling paper. The intra-assay precision for all analytes was <5% at 2000 nM, <7% at 750 nM and <10% at 100 nM. The interassay precision for all analytes was <4% at 2000 nM, <7% at 750 nM and <12% at 100 nM. The lower limit of quantitation was 100 nM for all analytes. The limit of detection was 30 nM in 100 μL venous blood applied to sampling paper.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Hatton C.; Peto T.; Bunch C.; Pasvol G.; Russel S.; Singer C.; Edwards G.; Winstanley P.Lancet 1986, 411–413.
Neftel, K.; Woodly, W.; Schmidt, R.; Frich P.; Fehr, J.Br. Med. J. 1986,292, 721–723.
Rhodes, E.G.H.; Ball, J.; Franklin, I.M.Br. Med. J. 1986,292, 717–718.
World Health Organization 19th Expert Committee on Malaria, Report. Geneva; WHO,1993.
Olliaro, P.; Nevill, C.; LeBras, J.; Mussano, P.; Garner, P.; Brasseur, P.Lancet 1996,348, 1196–1201.
Laurent, F.; Saivin, S.; Chretien, P.; Magnaval, J.F.; Peyron, F.; Sqalli, A.; Tufenkji, A.E.; Coulais, Y.; Baba, H.; Campistron, G.; Regis, H.; Ambroise-Thomas, P.; Bryskier, A.; Houin, G.;Arzneim.-Forsch./Drug Res. 1993,43, 612–616.
Churchill, F.C.; Patchen, L.C.; Cambell, C.C.; Schwartz, I.K.; Nguyen-Dinh, P.; Dickinson, C.M.Life Sci. 1985,36, 53–62.
Churchill, F.C.; Mount, D.L.; Patchen, L.C.; Björkman, A.J. Chromatogr. 1986,377, 307–318.
Mount, D.L.; Patchen, L.C.; Nguyen-Dinh, P.; Schwartz, I.K.; Barber, A.M.; Churchill, F.C.J. Chromatogr. 1986,383, 375–386.
Naisbit, D.J.; Ruscoe, J.E.; Williams, D.; O'Neill, P.M.; Pirmohamed, M.; Park, B.K.J. Pharmacol. Exp. Ther. 1997,280, 884–893.
Winstanley, P.A.; Edwards, I.G.; Orme, M.L.; Breckenridge, A.M.Br. J. Pharm. Pharmacol. 1987,23, 1–7.
Ducharme, J.; Farinotti, R.Clin. Pharmacokinet. 1996,31, 257–274.
Bergqvist, Y.; Domeij-Nyberg, B.J. Chromatogr. 1983,272, 137–148.
Rombo, L.; Ericsson, Ö.; Alva'n, G.; Lindström, B.; Gustafsson, L.L.; Sjöqvist, F.Ther. Drug Monit. 1985,7, 211–215.
Mihaly, G.W.; Nicholl, D.D.; Edwards, G.; Ward, S.A.; Orme, M.L'E.; Warrell, D.A.; Breckenridge, A.M.J. Chromatogr. 1985,337, 166–171.
Pussard, E.; Verdier, F.; Blayo, M.-C.; Pocidalo, J.-J.C. R. Acad. Sc. Paris 1985,301, 383–385.
Pussard, E.; Verdier, F.; Blayo, M.-C.J. Chromatogr. 1986,374, 111–118.
Brasseur, P.; Guiguemde, R.; Diallo, S.; Guiyedi, V.; Kombila, M.; Ringwald, P.; Olliaro, P.Trans. R. Soc. Trop. Med. Hyg. 1999,93, 645–650.
CDC HIV/Aids Prevention Newsletter, July1997.
Dadgar, D.; Burnett, P.E.; Choc, M.G.; Gallicano, K.; Hooper, J.W.J. Pharm. Biomed. Anal. 1995,13, 89–97.
Dua, V.K.; Kar, P.K.; Gupta, N.C.; Sharma, V.P.J. Pharm. Biomed. Anal. 1999,21, 199–205.
Hawley, S.R.; Bray, P.G.; Park, B.K.; Ward, S.A.Molecul. Biochem. Parasitol. 1996,80, 15–25.
ACD/pKa DB, version 1.2, Advanced Chemistry Development Inc., Toronto, Ontario, Canada.
Knudsen, R. C.; Slazyk, W.E.; Richmond, J.Y.; Hannon, W.H.Infant Screening 1993,16.
Lindegardh, N.; Funding, L.; Bergqvist, Y.J. Chromatogr. 2001,758, 137–144.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lindegårdh, N., Forslund, M., Green, M.D. et al. Automated solid-phase extraction for determination of amodiaquine, chloroquine and metabolites in capillary blood on sampling paper by liquid chromatography. Chromatographia 55, 5–12 (2002). https://doi.org/10.1007/BF02492307
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02492307