Summary
Limits of detection and quantification (LOD andLOQ) are two fundamental elements of method validation. Although strict statistical definitions exist, a variety of procedures based on different means of estimating the standard deviation of the blank can be used to evaluate these limits. An experiment set up to investigate their compatibility for an LC-UV method has proved they are not equivalent. We therefore recommend an alternative and absolute methodology:LOQ is obtained from the targetRSD chosen for the assay andLOD is taken as three tenths of theLOQ. To demonstrate the method is not restricted to LC-UV it has been applied to LC-MS and GC-FID.
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Vial, J., Le Mapihan, K. & Jardy, A. What is the best means of estimating the detection and quantification limits of a chromatographic method?. Chromatographia 57 (Suppl 1), S303–S306 (2003). https://doi.org/10.1007/BF02492120
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DOI: https://doi.org/10.1007/BF02492120