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Determination of vitamins A, D, E, and K in human and bovine serum, and β-carotene and vitamin A palmitate in cosmetic and pharmaceutical products, by isocratic HPLC

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Summary

An HPLC method has been developed for the determination of fat-soluble vitamins. Ten fat-soluble vitamins were separated simultaneously on a 25 cm×4.6 mm i.d. Hypersil C18 column with acetonitrile-dichloromethane-methanol, 60:20:20 (v/v) as mobile phase at 1.0 mL min−1 with wavelength-programmed ultraviolet-visible-absorbance detection. Total analysis time was 12 min. The limits of detection were 0.03, 0.01, 0.55, 1.84, 0.02, 0.02, 0.01, 0.16, 0.33, 0.01, and 0.01 ng mL−1 for retinol, retinyl acetate, retinyl palmitate, β-carotene, ergocalciferol, cholecalciferol, tocopherol, tocopherol acetate, phylloquinone, menatetrenone, and menadione, respectively. Analysis of human serum 2–7 days after ingestion of oral vitamins and Chinese herbs led to the conclusion that the concentration of vitamins was higher than for control serum.

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References

  1. Kaplan, L.A.; Pesce, A.J.Clinical Chemistry Theory, Analysis, and Correlation, 3rd Mosby Year Book,1996, p. 762.

  2. Takahashi, O.Toxicol. Lett. 1993,69, 257.

    Article  CAS  Google Scholar 

  3. Price, R.J.; Mistry, H.; Wield, P.T.; Renwick, A.B.; Beamand, J.A.; Lake, B.G.Arch. Toxicol. 1996,71, 107.

    Article  CAS  Google Scholar 

  4. Blanco, D.; Pajares, M.; Escotet, V.J.; Gutierrez, M.D.J. Liq Chromatogr. 1994,17, 4513.

    CAS  Google Scholar 

  5. Chase, G.W.; Akoh, C.C.; Eitenmiller, R.R.; Landen, W.O.J. Liq. Chromatogr. 1995,18, 3129.

    CAS  Google Scholar 

  6. Gomis, D.B.; Arias, V.E.; Alvarez, L.E.F.; Alvarez, M.D.G.Anal. Chim. Acta. 1995,315, 177.

    Article  Google Scholar 

  7. Zamarreno, M.M.D.; Perez, A.S.; Rodrignez, M.S.; Perez, M.C.G.; Mendes, J.H.Talanta 1996,43, 1555.

    Article  Google Scholar 

  8. Andreoli, P.; Careri, M.; Manini, P.; Mori, G.; Musci, M.Chromatographia 1997,44, 605.

    Article  CAS  Google Scholar 

  9. Gong, B.E.; Ho, J.W.J. Liq. Chromatogr. Related Technol. 1997,20, 2389.

    CAS  Google Scholar 

  10. Vliet, T.V.J. Chromatogr. 1991,553 179.

    Article  Google Scholar 

  11. Arnaud, J.; Forties, I.; Blachier, S.; Kia, D.; Favier, A.J. Chromatogr. 1991,572, 103.

    CAS  Google Scholar 

  12. Lee, B.L.; Chua, S.C.; Ong, H.Y.; Ong, C.N.J. Chromatogr. 1992,581, 41.

    CAS  Google Scholar 

  13. Epler, K.S.; Ziegler, R.G.; Craft, N.E.J. Chromatogr. 1993,619, 37.

    CAS  Google Scholar 

  14. Bui, M.H.J. Chromatogr. 1994,654, 129.

    CAS  Google Scholar 

  15. Cham, B.E.; Roeser, H.P.; Kamst, T.W.Clin. Chem. 1989,35, 2285.

    CAS  Google Scholar 

  16. Usui, Y.; Nishimura, N.; Kobayashi, N.; Okanoue, T.; Kimoto, M.; Ozawa, K.J. Chromatogr. 1989,489, 291.

    CAS  Google Scholar 

  17. Lambert, W.E.; Vanneste, L.; De Leenheer, A.P.Clin. Chem. 1992,38, 1743.

    CAS  Google Scholar 

  18. Guv, X.Q.; Zhao, Y.B.; Xu, J.G.Anal. Chim. Acta 1997,343, 109.

    Article  Google Scholar 

  19. Torro, I.G.; Mateo, J.V.G.; Calataynd, J.M.Analyst 1997,122, 139.

    Article  Google Scholar 

  20. Hollis, B.W.; Pittard, W.B.; Reinhardt, T.A.J. Clin. Endocrinol. Metab. 1986,62, 41.

    Article  CAS  Google Scholar 

  21. Amin, M.J. Liq. Chromatogr. 1988,11, 1347.

    CAS  Google Scholar 

  22. Watson, D.; Setchell, K.D.R.; Ross, R.Biomed. Chromatogr. 1991,5, 153.

    Article  CAS  Google Scholar 

  23. Aksnes, L.Scand. J. Clin. Lab. Invest. 1992,52, 177.

    CAS  Google Scholar 

  24. Haddad, J.G.; Matsuoka, L.Y.; Hollis, B.W.; Hu, Y.Z.; Wortsman, J.J. Clin. Invest. 1993,91, 2552.

    Article  CAS  Google Scholar 

  25. Paul, M.M.; Van, H.; Roel, E.; Tjeerd, P.Biomed. Chromatogr. 1987,2, 79.

    Article  Google Scholar 

  26. Nelis, H.J.C.F.; De Leenheer, A.P.Anal. Chem. 1983,55, 270.

    CAS  Google Scholar 

  27. Gatautis, V.; Pearson, K.H.Clin. Chim. Acta. 1987,166, 195.

    Article  CAS  Google Scholar 

  28. Driskell, W.J.; Bashor, M.M.; Nee Se, J.W.Clin. Chem. 1983,29, 1042.

    CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Applied Chemistry, China Nan University of Pharmacy and Science, Tainan, Taiwan 71710, ROC

    Lai-Hao Wang & Shin-Hung Huang

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  1. Lai-Hao Wang
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  2. Shin-Hung Huang
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Wang, LH., Huang, SH. Determination of vitamins A, D, E, and K in human and bovine serum, and β-carotene and vitamin A palmitate in cosmetic and pharmaceutical products, by isocratic HPLC. Chromatographia 55, 289–296 (2002). https://doi.org/10.1007/BF02491661

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  • DOI: https://doi.org/10.1007/BF02491661

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