Summary
Columns for open-tubular (OT) capillary electrochromatography (CEC), coated with 1,4,7,10-tetraazacyclotridecane-11,13-dione (dioxo[13]aneN4) by use of the sol-gel technique, have been investigated for the first time. Dioxol[13]aneN4 was reacted with 3-(2-cyclooxypropoxy)propyltrimethoxysilane and the product was then mixed with tetraethoxysilane (TEOS) and water to form a glass matrix as a network in which the stationary phase (dioxo[13]aneN4) was incorporated. In comparison with OTCEC columns prepared by the sol-gel process with TEOS only, the sol-gel-derived macrocyclic dioxopolyamine columns enabled better separations of a mixture of isomeric nitrophenols and benzenediols, a mixture of isomeric aminophenols and diaminobenzenes, and a mixture of four biogenic monoamine neurotransmitters. High efficiencies (60 000—340 000 plates m−1) were achieved for isomeric benzenediols, aminophenols, nitrophenols, and diaminobenzenes. Migration time and theoretical plate number reproducibility was satisfactory;RSD was <2% for one column and <8.5% from column to column.
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Wang, Y.C., Zeng, Z.R., Xie, C.H. et al. Use of the sol-gel technique to prepare capillary columns coated with a macrocyclic dioxopolyamine for open-tubular capillary electrochromatography. Chromatographia 54, 475–479 (2001). https://doi.org/10.1007/BF02491202
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DOI: https://doi.org/10.1007/BF02491202