Skip to main content
SpringerLink
Log in

Application of a chromolith speedROD RP-18e HPLC column: Determination of ochratoxin A in different wines by high-performance liquid chromatography-tandem mass spectrometry

  • Short Communications
  • Column Liquid Chromatography
  • Published:
Chromatographia Aims and scope Submit manuscript

Summary

Two different types of HPLC column have been used to determine the mycotoxin ochratoxin A in red and white wines by liquid chromatography-tandem mass spectrometry (LC-MS-MS). The performance of a 12.5-cm standard particle-based column and a 5-cm monolithic type column (Merck Chromolith SpeedROD) was compared. Sample preparation consisted of a simple, single solid-phase extraction step. By use of MS detection in multiple reaction monitoring (MRM) mode a limit of determination of 0.5 ppb was achieved for both chromatographic arrangements. The monolithic column was found to give comparable results while enabling higher flow rates; this reduced analysis time by a factor of three.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Nakanishi, K.; Soga, N.J. Non Cryst. Solids 1992,139, 1.

    Article  CAS  Google Scholar 

  2. Nakanishi, K.; Soga, N.J. Non Cryst. Solids 1992,139, 14.

    Article  CAS  Google Scholar 

  3. Nakanishi, K.J. Porous Mater. 1997,4, 67.

    Article  CAS  Google Scholar 

  4. Minakuchi, H.; Nakanishi, K.; Soga, N.; Tanaka, N.J. Chromatogr. A 1997,762, 135.

    Article  CAS  Google Scholar 

  5. Ishizuka, N.; Minakuchi, H.; Nakanishi, K.; Soga, N.; Tanaka, N.J. Chromatogr. A 1998,797, 133.

    Article  CAS  Google Scholar 

  6. Cabrera, K.; Wieland, G.; Lubda, D.; Nakanishi, K.; Soga, N.; Minakuchi, H.; Unger, K.K.Trends Anal. Chem. 1998,1, 50.

    Article  Google Scholar 

  7. Krogh, P. (Ed.)Mycotoxins in Food, Academic Press, London,1987.

    Google Scholar 

  8. van Egmond, H.FAO Food and Nutrition Paper, Advanced Copy, p. 7.

Download references

Author information

Authors and Affiliations

  1. Institute of Analytical Chemistry, University of Vienna, Währinger Straße 38, 1090, Vienna, Austria

    P. Zöllner, A. Leitner & W. Lidner

  2. Merck KGaA, Frankfurter Str. 250, 64271, Darmstadt, Germany

    D. Lubda & K. Cabrera

Authors
  1. P. Zöllner
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Leitner
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. Lubda
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. K. Cabrera
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. W. Lidner
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zöllner, P., Leitner, A., Lubda, D. et al. Application of a chromolith speedROD RP-18e HPLC column: Determination of ochratoxin A in different wines by high-performance liquid chromatography-tandem mass spectrometry. Chromatographia 52, 818–820 (2000). https://doi.org/10.1007/BF02491011

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02491011

Key Words

Search

Navigation