Skip to main content
SpringerLink
Log in

Enantioseparation of venlafaxine andO-desmethylvenlafaxine by capillary electrophoresis with mixed cyclodextrins

  • Short Communications
  • Published:
Chromatographia Aims and scope Submit manuscript

Summary

Capillary electrophoresis (CE) has been successfully applied to the separation of the enantiomers of venlafaxine (Vx) and its main active metabolite,O-desmethylvenlafaxine (ODV), by use of a mixture of two cyclodextrins (CDs) added to the background electrolyte (BGE). The use of carboxymethylated β-cyclodextrin (CMB) enabled the enantioseparation of both Vx and ODV, although separation of all four enantiomers was not achieved. Addition of a second cyclodextrin (α-CD) to the BGE resulted in the simultaneous resolution of the enantiomers of both compounds. In this system, α-CD enabled discrimination between Vx and ODV whereas CMB enabled the enantioseparation. Resolution was strongly influenced by the ratio of the concentrations of the two complexing agents. Baseline resolution of Vx and ODV was achieved in an uncoated capillary. Addition of organic modifiers to the BGE had a substantial effect on the interaction of the analytes with the α-CD.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. W.A. Morton, S.C. Sonne, M.A. Verga, Ann. Pharmacother.29, 387 (1995).

    CAS  Google Scholar 

  2. R.L. Vu, D. Helmeste, L. Albers, C. Reist, J. Chromatogr. B.703, 195 (1997).

    CAS  Google Scholar 

  3. S.E. Ball, D. Ahem, J. Scatina, J. Kao, Br. J. Clin. Pharm.43, 619 (1997).

    Article  CAS  Google Scholar 

  4. S.V. Otton, S.E. Ball, S.W. Cheung, T. Inaba, R.L. Rudolph, E.M. Sellers, Br. J. Clin. Pharmacol.41, 149 (1996).

    Article  CAS  Google Scholar 

  5. S.M. Holliday, P. Benfield, Drugs49, 280 (1995).

    CAS  Google Scholar 

  6. K.J. Klamerus, K. Maloney, R.L. Rudolph, S.F. Sisenwine, W.J. Jusko, S.T. Chiang, J. Clin. Pharmacol.32, 716 (1992).

    CAS  Google Scholar 

  7. D.R. Hicks, D. Wolianuk, A. Rusell, N. Cavanaugh, M. Krami, Ther. Drug Monit.16, 100 (1994).

    CAS  Google Scholar 

  8. D. Albano, J. Amchin, Am. J. Health-Syst. Pharm.52, 1573 (1995).

    CAS  Google Scholar 

  9. B. Levine, A.J. Jenkins, M. Quenn, R. Jufer, J.E. Smialek, J. Anal. Toxicol.20, 502 (1996).

    CAS  Google Scholar 

  10. A.T. Parsons, R.M. Anthony, J.E. Meeker, J. Anal. Toxicol.20, 266 (1996).

    CAS  Google Scholar 

  11. C. Long, J. Crifasi, D. Maginn, M. Graham, S. Teas, J. Anal. Toxicol.21, 166 (1997).

    CAS  Google Scholar 

  12. E.M. Clement, J. Odontiadis, M. Franklin, J. Chromatogr. B.705, 303 (1998).

    CAS  Google Scholar 

  13. P.C. Wang, S.R. Howell, J. Scatina, S.F. Sisenwine, Chirality4, 84 (1992).

    Article  Google Scholar 

  14. S. Fanali, V. Cotichini, R. Porrà, J. Cap. Electroph.4, 21 (1997).

    CAS  Google Scholar 

  15. S. Fanali, C. Desiderio, Z. Aturki, J. Chromatogr. A.772, 185 (1997).

    Article  CAS  Google Scholar 

  16. K.W. Whitaker, C.L. Copper, M.J. Sepaniak, J. Microcol. Sep.8, 461 (1996).

    Article  CAS  Google Scholar 

  17. S. Fanali, E. Camera, Chromatographia43, 247 (1996).

    Article  CAS  Google Scholar 

  18. B. Chankvetadze, J. Chroamtogr. A792, 269 (1997).

    Article  CAS  Google Scholar 

  19. B. Chankvetadze, G. Endresz, G. Blaschke, Electrophoresis15, 804 (1994).

    Article  CAS  Google Scholar 

  20. Y. Tanaka, M. Yanagawa, S. Terabe, J. High Resol. Chromatogr.19, 421 (1996).

    Article  CAS  Google Scholar 

  21. V.C. Anibogu, C.L. Copper, M.J. Sepaniak, J. Chromatogr. A.705, 343 (1995).

    Article  Google Scholar 

  22. L.S. Lurie, F.X. Klein, J.A. Dal Cason, M.J. LeBelle, R. Brenneisen, R.E. Weinberger, Anal. Chem.66, 4019 (1994).

    Article  CAS  Google Scholar 

  23. M. Fillet, P. Hubert, J. Crommen, Electrophoresis18, 1013 (1997).

    Article  CAS  Google Scholar 

  24. L.S. Lurie, J. Chromatogr. A.792, 297 (1997).

    Article  CAS  Google Scholar 

  25. J. Wang, I.M. Warner, J. Chromatogr. A.711, 297 (1995).

    Article  CAS  Google Scholar 

  26. F. Lelièvre, P. Gareil, Y. Bahaddi, H. Galons, Anal. Chem.69, 369 (1997).

    Google Scholar 

  27. M. Fillet, I. Bechet, J. Crommen, P. Hubert, J. Pharmaceut. Biomed. Anal.14, 1107 (1996).

    Article  CAS  Google Scholar 

  28. B.J. Spencer, W. Zhang, W.C. Purdy, Electrophoresis18, 736 (1997).

    Article  CAS  Google Scholar 

  29. T. Schmitt, H. Engelhardt, J. High Resol. Chromatogr.16, 525 (1993).

    Article  CAS  Google Scholar 

  30. T. Schmitt, H. Engelhardt, Chromatographia.37, 475 (1993).

    Article  CAS  Google Scholar 

  31. S.A.C. Wren, J. Chromatogr.636, 57 (1993).

    Article  CAS  Google Scholar 

  32. C. Quang, M.G. Khaledi, J. Chromatogr. A.692, 253 (1995).

    Article  CAS  Google Scholar 

  33. I. Bechet, P. Paques, M. Fillet, P. Hubert, J. Crommen, Electrophoresis15, 818 (1994).

    Article  CAS  Google Scholar 

  34. S. Cladrowa-Runge, R. Hirz, E. Kenndler, A. Rizzi, J. Chromatogr. A.710, 339 (1995).

    Article  CAS  Google Scholar 

  35. S.A.C. Wren, R.C. Rowe, J. Chromatogr.609, 363 (1992).

    Article  CAS  Google Scholar 

  36. S. Naylor, L.M. Benson, A.J. Tomlinson, J. Cap. Electroph.1, 181 (1994).

    CAS  Google Scholar 

  37. P.D. Ferguson, D.M. Goodall, J.S. Loran, J. Chromatogr. A.745, 25 (1996).

    Article  CAS  Google Scholar 

  38. S. Fanali, Z. Aturki, J. Chromatogr. A.694, 297 (1995).

    Article  CAS  Google Scholar 

  39. S. Fanali, J. Chromatogr.545, 437 (1991).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Istituto di Cromatografia del C.N.R., Area della Ricerca di Roma, P.O. Box 10, 00016, Monterotondo Scalo (Roma), Italy

    S. Rudaz, C. Desiderio & S. Fanali

  2. Laboratory of Pharmaceutical Analytical Chemistry, University of Geneva, 20 Bd d’Yvoy, 1211, Geneva 4, Switzerland

    J. -L. Veuthey

Authors
  1. S. Rudaz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. -L. Veuthey
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. Desiderio
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. Fanali
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rudaz, S., Veuthey, J.L., Desiderio, C. et al. Enantioseparation of venlafaxine andO-desmethylvenlafaxine by capillary electrophoresis with mixed cyclodextrins. Chromatographia 50, 369–372 (1999). https://doi.org/10.1007/BF02490844

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02490844

Key Words

Search

Navigation