Abstract
Pseudomonas aeruginosa is an opportunistic pathogen that infects compromised hosts. It is therefore important to clarify the bactericidal activity of antimicrobial agents against this bacterium. We evaluated the antimicrobial potency of several antibiotics againstP. aeruginosa by measuring the MIC and the minimum bactericidal concentration (MBC). Imipenem, panipenem meropenem, ceftazidime, cefpirome, cefepime, and cefozopran were the antibiotics evaluated. The 40 strains ofP. aeruginosa used in this analysis were isolated from the sputum of 40 patients. The MIC was determined by the microbroth dilution method. Afterwards, we determined the MBC, which is the lowest concentration of antibiotic at which all bacteria in the diluted culture are killed. The MIC and MBC of meropenem was determined to be 2 to 8 times lower and 2 to 4 times lower, respectively, than those of other carbapenem antibiotics tested. The MIC and MBC of cefozopran were determined to be 2 to 16 times lower and 4 to 16 times lower, respectively, than those of other cephem antibiotics tested. Meropenem and cefozopran show the greatest bactericidal activity againstP. aeruginosa among the, carbapenem and cephem antibiotics tested. Meropenem would be one of the best selections for treatment of pyocyanic infections in immunocompromised patients, because it also provides a postantibiotic effect againstP. aeruginosa.
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References
Nakae T. Role of membrane permeability in determining antibiotic resistance inPseudomonas aeruginosa. Microbiol Immunol 1995;39:221–229.
Tsuji A, Kaneko Y, Yamaguchi K, Goto S. Correlation between the in vitro and in vivo effects of fourteen beta-lactam compounds in mice with systemic infection. Chemotherapy (Basel) 1994;40:324–332.
Japan Society of Chemotherapy. Standard method for dilution antimicrobial susceptibility tests for bacteria (microdilution method) (in Japanese). Chemotherapy (Tokyo) 1990;38:103–105.
Jun I, Matsuhisa I, Takeshi N. Survey of the sensitivities of clinical isolates to antibacteria agents (annual report) (in Japanese). Jpn J Antibiot 1998;51 (2):47–68.
Sakai C, Takagi T, Murata N, Tanaka N. Statistical and clinical study ofPseudomonas aeruginosa bacteremia complicating neoplastic disease (in Japanese). Jpn J Cancer Clin 1996;42:591–595.
Sumita Y, Fukasawa M. Meropenem resistance inPseudomonas aeruginosa. Chemotherapy 1996;42:47–56.
Sumita Y, Fukasawa M, Okuda T. Comparison of two carbapenems, SM-7338 and imipenem: affinities for penicllin-binding proteins and morphological changes. J Antibiot 1990;43:314–320.
Sunagawa M, Matsumura H, Sasaki A, Yamaga H, Sumita Y, Nouda H. Structure-activity relationship of 1 β-methylcarbapenem to its antibacterial activity: effect of the C-2 side chain and the 1 β-methyl group. J Antibiot 1996;49:1175–1178.
Kuwahara-Arai K, Yokota T. In vitro antibacterial activity of cefozopran (in Japanese). Chemotherapy 1993;41 (S-4): 11–19.
Nadler HL, Pitkin DH, Sheikh W. The postantibiotic effect of meropenem and imipenem on selected bacteria. J Antimicrob Chemother 1989;24(S-A):S225-S231.
Sumita Y, Fukasawa M. The increased antipseudomonal activity of meropenem in minimal medium (in Japanese). Chemotherapy (Tokyo) 1995;43:393.
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Nishizawa, K., Hirano, M., Kimura, A. et al. Evaluation of the antimicrobial activity of carbapenem and cephem antibiotics againstPseudomonas aeruginosa isolated from hospitalized patients. J Infect Chemother 4, 174–176 (1998). https://doi.org/10.1007/BF02490163
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DOI: https://doi.org/10.1007/BF02490163