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Biocompatibility of MPC: in vivo evaluation for clinical application

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Abstract

Biocompatibility is important to assure a mild body reaction to an implanted device and its long-term stability and functionality. In diabetes research, subcutaneously implanted glucose monitoring systems need biocompatible surfaces for long-term application. The biocompatibility of poly(2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate) (MPC), a material similar to the phospholipid layer of a cell membrane, was compared in vivo with the biocompatibility of polyurethane (PU), polyvinyl alcohol (PVA), and cuprophane (CUP). Needle-type glucose sensors and hollow-fiber probes used for microdialysis were coated with these four different biomaterials and implanted subcutaneously in 18 rats and 7 healthy volunteers. At set intervals, the implants and, in the case of the rats, also the surrounding tissue were removed and characterized by light and electron microscopy. MPC-coated sensors and hollow-fiber probes showed smooth and thin deposits in flat layers, whereas the surface deposits on PU- and PVA-coated sensors and those on CUP hollow-fiber probes appeared as rough, irregular, and dense attachments of aggregated cells and protein. This study confirmed results from earlier in vitro tests by showing the biocompatibility and reliability of MPC. Even though the amount of protein and cells attached to the MPC surface was not as low as expected from in vitro experiments, the biocompatibility and long-term stability of the implanted devices were superior to those of PU, PVA, and CUP.

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Authors and Affiliations

  1. Department of Metabolic Medicine, Kumamoto University School of Medicine, 1-1-1 Honjo, 860-8556, Kumamoto, Japan

    Tanja Nowak PhD, Kenro Nishida MD, PhD, Seiya Shimoda MD, PhD, Yumi Konno MD, Kenshi Ichinose, Michiharu Sakakida MD, PhD & Motoaki Shichiri MD, PhD

  2. Institute for Medical and Dental Engineering, Tokyo Medical and Dental University, Tokyo, Japan

    Nobuo Nakabayashi PhD

  3. Department of Materials Science, Faculty of Engineering, University of Tokyo, Tokyo, Japan

    Kazuhiko Ishihara PhD

Authors
  1. Tanja Nowak PhD
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  2. Kenro Nishida MD, PhD
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  3. Seiya Shimoda MD, PhD
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  4. Yumi Konno MD
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  5. Kenshi Ichinose
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  6. Michiharu Sakakida MD, PhD
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  7. Motoaki Shichiri MD, PhD
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  8. Nobuo Nakabayashi PhD
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  9. Kazuhiko Ishihara PhD
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Corresponding author

Correspondence to Kenro Nishida MD, PhD.

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Nowak, T., Nishida, K., Shimoda, S. et al. Biocompatibility of MPC: in vivo evaluation for clinical application. J Artif Organs 3, 39–46 (2000). https://doi.org/10.1007/BF02479925

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  • DOI: https://doi.org/10.1007/BF02479925

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