Abstract
The effects of cortexin, epithalamin, and synthetic peptides on the growth of processes in sensory neurons and on the development of fragments of cortical and subcortical brain structures were studied in organotypic cultures from 10-11-day chick embryos. Cortexin (20 and 100 ng/ml), epithalamin (20 and 200 ng/ml), polypeptides M and P (2 and 20 ng/ml) had neurite-stimulating actions, evident on day 3 of dorsal root ganglion culture. Addition of cortexin (100 ng/ml) or polypeptide M (20 ng/ml) to the culture medium of cerebral cortex explants stimulated explant development. Addition of cortexin at the same concentration to explants of subcortical formations suppressed their development. Epithalamin (200 ng/ml) or polypeptide M (100 ng/ml) stimulated the development of explants from subcortical formations, the existence of the neurite-stimulating effect effects of these cytokines provided the basis for identifying the mechanism of action of brain peptides.
Similar content being viewed by others
References
G. N. Akoev and N. I. Chalisova, “Neurotrophic factors from the CNS,”Uspekhi Fiziol. Nauk.,21, No. 4, 138–142 (1990).
N. B. Gorbunova and V. N. Kalyunov, “Nerve growth factor in different types of stress in experimental conditions,”Izv. Akad. Nauk Belarus SSR,3, 85–89 (1993).
V. P. Goncharova, N. I. Chalisova, A. V. Romanyuk, and G. N. Akoev, “The neurite-stimulating activity of a protein isolated from the lysosomal fraction of brain tissue in tissue cultures,”Dokl. Akad. Nauk SSSR,285, No. 5, 1238–1241 (1985).
V. N. Kalyunov,The Biology of Newe Tissue Growth Factor [in Russian], Nauka i Tekhnika, Minsk (1986).
V. N. Morozov and V. Kh. Khavinson, “A new class of biological regulators of multicellular systems—cytomedins,”Uspekhi Sovrem. Biologii,96, No. 3, 339–346 (1983).
V. Kh. Khavinson, “Review of studies and applications of peptide bioregulators in gerontology,” in:Proceedings of the International Symposium “Gerontological Aspects of the Peptide Regulation of Body Function” [in Russian], Nauka, St. Petersburg (1996) pp. 84–85.
V. Kh. Khavinson, V. G. Morozov, and B. I. Kuznik, “Cytomedins and their role in regulating physiological functions,”Uspekhi Sovrem. Biologii,115, No. 3, 353–367 (1986).
N. I. Chalisova, V. F. Mel'kishev, G. N. Akoev, M. I. Lyudyno, and T. F. Kurenkova, “The stimulating effect of prolactin on neurite growth in sensory cells in organotypic cultures,”Tsitologiya,33, No. 2, 29–31 (1991).
N. I. Chalisova, S. L., Yatsuk, and M. I. Lyudyno, “Studies of the neurite-stimulating activity of cerebrospinal fluid in children,” in:Proceedings of the Conference “Methods of Overcoming Pediatric Neurological Invalidity” [in Russian], Samara (1993), p. 27.
Y. A. Barde, D. Edgar, and H. Thoenen, “Purification of a new neurotrophic factor from mammalian brain,”Bur. Mol. Biol.,1, 549–553 (1988).
T. Ebendal, “Function and evolution in the NGF family and its receptors,”J. Neurosci. Res.,32, No. 1, 461–470 (1992).
A. G. Glasky, R. F. Ritzman, C. Melchion, and J. Thomas, “AIT-82 modulates neuritogenesis through a carbon monoxide/guanylate cyclase mechanism and restores age-induced memory deficits,”Soc. Neurosci. Abstr.,20, No. 2, 1099 (1994).
V. Hamburger and H. Hamilton, “A series of normal stages in the development of the chick embryo,”J. Morphol.,88, No. 1, 49–92 (1951).
R. Klein, D. Martin-Zanca, H. Barbacid, and H. Thoenen, “Expression of the tyrosine kinase receptor gene trkB is confined to the murine embryonic and adult nervous system,”Development,109, No. 1, 845–850 (1990).
S. Korshing, G. Auberger, and R. Heumann, “Levels of nerve growth factor (NGF) and its mRNA in the central nervous system of the rat correlate with cholinergic innervation,”EMBO J.,4, 1389–1393 (1985).
R. Levi-Montalcini and P. Angeletty, Nerve growth factor,”Physiol. Rev.,48, 543–569 (1968).
Author information
Authors and Affiliations
Additional information
Translated from Rossiiskii Fiziologicheskii Zhurnal imeni I. M. Sechenova, Vol. 85, No. 1, pp. 29–35, January, 1999.
Rights and permissions
About this article
Cite this article
Chalisova, N.I., Khavinson, V.K. Studies of cytokines in nerve tissue cultures. Neurosci Behav Physiol 30, 261–265 (2000). https://doi.org/10.1007/BF02471779
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02471779