Abstract
In vitro germination of a myco-heterotrophic orchid,Erythrorchis ochobiensis, was tested in the presence of ectomycorrhizal fungi,Lyophyllum shimeji andTricholoma fulvocastaneum. Lyophyllum shimeji stimulated the germination after incubation for 1.5 mo. Although most germinated seeds did not grow further after 3 mo, several seeds developed into small protocorms but showed amorphous profiles. Fungal mycelia were observed in the germinated seeds and protocorms, but pelotons were not detected. Since the seeds did not germinate axenically, it may be suggested that the fungus has the ability to stimulate germination.
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Reterature cited
Björkman, E. 1960.Monotropa hypopitys L., an epiparasite on tree roots. Physiol. Plant.97: 401–411.
Campbell, E. O. 1963.Gastrodia minor Petrie, an epiparasite of manuka. Trans. Roy. Soc. N. Z. Bot.2: 73–81.
Garay, L. A. 1986. Olim Vanillaceae. Botanical Museum Leaflets, Harvard Univ.30: 223–237.
Imazeki, R., Otani, Y. and Hongo, T. 1988. Fungi of Japan, pp. 13, 25–53. Yama-kei Publishers, Tokyo. (In Japanese.)
Mori, K., Hamaya, E., Shimomura, T. and Ikegami, Y. 1969. Production of virus-free plants by means of meristem culture. J. Cent. Agr. Expt. Sta.13: 45–110. (In Japanese.)
Ogawa, M. 1977. Microbial ecology of ‘Shiro’ inTricholoma matsutake (S. Ito et Imai) Sing. and its allied species. VI.Tricholoma fulvocastaneum inQuercus serrata-Quercus acutissima forest. Trans. Myco. Soc. Japan.18: 286–297.
Ogawa, M. 1978. Microbial ecology of ‘Shiro’ inTricholoma matsutake (S. Ito et Imai) Sing. and its allied species. VII.Tricholoma fulvocastaneum Hongo inCastanopsis cuspidata forest. Trans. Mycol. Soc. Japan.19: 37–46. (In Japanese.)
Smith, S. E. and Read, D. J. 1996. Mycorrhizal symbiosis, 2nd ed., pp. 350–353. Academic Press, London.
Tashima, Y., Terashita, T., Umata, H. and Matsumoto, M. 1978. In vitro development from seed to flower inGastrodia verrucosa under fungal symbiosis. Trans. Mycol. Soc. Japan19: 449–453.
Umata, H. 1995. Seed germination ofGaleola altissima, an achlorophyllous orchid, with aphyllophorales fungi. Mycoscience36: 369–372.
Umata, H., Kanetani, S. and Mori, T. 1994. The habitat and habit of a chlorophyll-free orchid,Erythrorchis altissima (Bl.) Bl. Acta Phytotax. Geobot.45: 131–138. (In Japanese.)
Warcup, J. H. 1985.Rhizanthella gardneri (Orchidaceae), itsRhizoctonia endophyte and close association withMelaleuca unicinata (Mytraceae) in Western Australia. New Phytol.99: 273–280.
Warcup, J. H. 1991. TheRhizoctonia endophytes ofRhizanthella (Orchidaceae). Mycological Research95: 656–659.
Zelmer, C. D. and Currah, R. S. 1995. Evidence for a fungal liaison betweenCorallorhiza trifida (Orchidaceae) andPinus contorta (Pinaceae). Can. J. Bot.73: 862–866.
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Umata, H. In vitro germination ofErythrorchis ochobiensis (Orchidaceae) in the presence ofLyophyllum shimeji, an ectomycorrhizal fungus. Mycoscience 38, 355–357 (1997). https://doi.org/10.1007/BF02464097
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DOI: https://doi.org/10.1007/BF02464097