Skip to main content
Log in

Morphological and phylogenetic studies onCordyceps sinensis distributed in southwestern China

  • Original Papers
  • Published:
Mycoscience

Abstract

Cordyceps is one of the target genera for modern mycological studies. Among themCordyceps sinensis is the most famous but poorly defined species because the fungus is endemic in districted regions of east Eurasia. We have explored the various growing regions and habitats where the fungus grows in the wild. We also examined authentic cultures for the species. We analyzed the sequences of ITS1, 2 and 5.8 S rDNA regions ofC. sinensis materials collected from 11 localities of southwestern China. Phylogenetic analyses were performed with these sequences and with additional sequences obtained from GenBank. All sequences formed a single cluster, which comprised two subgroups. Our results strongly suggested that intraspecific variation was rather small and that some species that are morphologically similar toC. sinensis but with different names might be synonymous withC. sinensis. The difference in the pharmaceutical activity among these collectedC. sinensis from different regions will be studied in the future.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Literature cited

  • Chaudhuri, H. 1931. Note on aCordyceps from Tibet. Trans. Br. Mycol. Soc.16: 203–205.

    Article  Google Scholar 

  • Chen, Y., Zhang, Y.-P., Yang, Y. and Yang, D. 1999. Genetic diversity and taxonomic implication ofCordyceps sinensis as revealed by RAPD markers. Biochemical Genetics37: 201–213.

    Article  PubMed  CAS  Google Scholar 

  • Felsenstein, J. 1993. PHYLIP Ver. 3.572c. Dept. Genetics, Univ. of Washington, Seatle.

    Google Scholar 

  • Gu, H., Liang, M., Zhao, G. and Zhao, Y. 1993. The first report on the investigation ofCordyceps variety in Jilin province. Studies and application of entomogenous fungi in China. vol. III, pp. 224–225. China Agic. Sci. Press. Beijing (In Chinese.)

    Google Scholar 

  • Hodge, K. T., Krasnoff, S. B. and Humber, R. A. 1996.Tolypocladium inflatum is the anamorph ofCordyceps subsessils. Mycologia88: 715–719.

    Google Scholar 

  • Kinjo, N., Mineshita, S., Wang, L. and Zang, M. 1996. Pharmacological effects of the mycelial extracts of cultured Tochu-kaso on the behabior and liver function in rat. Proceedings of Asian International Mycological Congress '96 Chiba, Japan, p. 68.

  • Kinjo, N. and Zang, M. 2001. Morphological and rDNA sequences ofCordyceps Sinensis in Himalaya Mountains. Bull. Coll. Liberal Art and Sci., Tokyo Dental and Medical University31: 59–72. (In Japanese.)

    Google Scholar 

  • Kobayasi, Y. 1941. The genusCordyceps and its allies. Sci. Reports Tokyo Bunrika Daigaku, Sect. B5(84): 53–260.

    Google Scholar 

  • Kornerup, A. and Wanscher, J. L. 1978. Methuen handbook of colour, 3rd ed. Eyre Methuen, London.

    Google Scholar 

  • Liang, Z., Liu, A. and Liu, Z. 1995. The development of ascospores inCordyceps sinensis (Berk.) Sacc. Acta Mycologica Sinica14(2): 148–152.

    Google Scholar 

  • Li, Shin.-Zhen. 1578. Compendium Materia Medica, vol. 41, Insects—3. (Ming dynasty, in classical Chinese.)

  • Liu, B. 1978. Chinese medical fungi 2nd ed. pp. 24–26. Shanshi People's Press, Shanshi (In Chinese.)

    Google Scholar 

  • Liu, Z.-Y., Yao, Y.-J., Liang, Z. Q., Pegler, D. N. and Chase, M. W. 2001. Molecular evidence for the anamorph-teleomorph connection inCordyceps sinensis. Mycol. Res.105: 827–832.

    Article  CAS  Google Scholar 

  • Rayner, R. W. 1970. A mycological colour chart. Commonwealth Mycological Institute. Kew and British Mycological Society.

  • Saitou, N. and Nei, M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evolution4: 406–425.

    CAS  Google Scholar 

  • Saccardo, P. A. 1878. Enumeratio pyrenomycetum hypocreaceorum huscusque congnitorum systeate xarpologico dispositorum. Michelia1: 277–325.

    Google Scholar 

  • Thompson, J. D., Higgins, D. G. and Gibson, T. J. 1994. Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucleic Acids Research22: 4673–4680.

    PubMed  CAS  Google Scholar 

  • Zang, M. and Kinjo, N. 1996. Type study on theCordyceps sinensis. Acta Botanica Yunnanica17: 63–65. (In Chinese with English summary.)

    Google Scholar 

  • Zang, M., Yang, D.-R. and Li, C.-D. 1990. A new taxon in the genusCordyceps from China. Mycotaxon37: 57–62

    Google Scholar 

  • Zhang, Y.-W., Zhang, K., Wang, C. and Yan, M. 1989. A new species ofCordyceps from Gansu, China. Trans. Mycol. Soc. Japan30: 295–299.

    Google Scholar 

  • Ying, J. and Zang, M. (eds.) 1994. Economic macrofungi from southwestern China. pp. 12–18. Pl. 16. Sci. Press, Beijing. (In Chinese.)

    Google Scholar 

  • Yang, D., Li, C., Yang, Y. and Shu, C. 1994. Entomogenous fungi in alpine region of Yunnan. Studies and application of entomogenous fungi in China. Vol. III, pp. 199–202. China Agic. Sci. Press. Beijing. (In Chinese.)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

About this article

Cite this article

Kinjo, N., Zang, M. Morphological and phylogenetic studies onCordyceps sinensis distributed in southwestern China. Mycoscience 42, 567–574 (2001). https://doi.org/10.1007/BF02460956

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02460956

Key Words

Navigation