Summary
The induction of mesoderm is an important process in early amphibian development. In recent studies, activin has become an effective candidate for a natural mesoderm-inducing factor. In the present study, we show that follistatin, an activin-binding protein purified from porcine ovary, inhibits the mesoderm-inducing activity of recombinant human activin A (rh activin A), which is identical to the erythroid differentiation factor (EDF). The quantity of follistatin required for effective suppression of activin was more than three-fold that of activin (w:w). Follistatin also inhibited the mesoderm-inducing activity of the vegetalizing factor purified from chick embryos, suggesting that the vegetalizing factor is closely related to activin.
Similar content being viewed by others
References
Asashima M (1975) Inducing effects of the presumptive endoderm of successive stages inTriturus alpestris. Rouxs Arch Dev Biol 177:301–308
Asashima M, Shimada K, Nakano H, Kinoshita K, Ueno N (1989) Mesoderm induction by activin A (EDF) inXenopus early embryo. Cell Differ Dev 27: Suppl 53
Asashima M, Nakano H, Shimada K, Kinoshita K, Ishii K, Shibai H, Ueno N (1990a) Mesodermal induction in early amphibian embryos by activin A (erythroid differentiation factor). Rouxs Arch Dev Biol 198:330–335
Asashima M, Nakano H, Uchiyama H, Davids M, Plessow S, Loppnow-Blinde B, Hoppe P, Dan H, Tiedemann H (1990b) The vegetalizing factor belongs to a family of mesoderm-inducing proteins related to erythroid differentiation factor. Naturwissenschaften 77:389–391
Carrol RS, Corrigan AZ, Gharib SD, Vale W, Chin WW (1989) Inhibin, activin and follistatin: regulation of follicle-stimulating hormone messenger ribonucleic acid levels. Mol Endocrinol 3:1969–1976
Esch FS, Shimasaki S, Mercado M, Cooksey K, Ling N, Ying S-Y, Ueno N, Guillemin R (1987) Structural characterization of follistatin: A novel follicle-stimulating hormone release-inhibiting polypeptide from the gonad. Mol Endocrinol 1:849–855
Eto Y, Tsuji T, Takezawa M, Takano S, Yokogawa Y, Shibai H (1987) Purification and characterization of erythroid differentiation factor (EDF) isolated from human leukemia cell line THP-1. Biochem Biophys Res Commun 142:1095–1103
Geithe H-P, Asashima M, Asashi K, Born J, Tiedemann H, Tiedemann H (1981) A vegetalizing inducing factor. Isolation and chemical properties. Biochem Biophys Acta 676:350–356
Green JBA, Howes G, Symes K, Cooke J, Smith JC (1990) The biological effects of XTC-MIF: quantitative comparison withXenopus bFGF. Development 108:173–183
Grunz H, McKeehan WL, Knochel W, Born J, Tiedemann H, Tiedemann H (1988) Induction of mesodermal tissues by acidic and basic heparin binding growth factors. Cell Differ 22:183–190
Grunz H, Born J, Davids M, Hoppe P, Loppnow-Blinde B, Tacke L, Tiedemann H, Tiedemann H (1989) A mesoderm-inducing factor from aXenopus cell line: chemical properties and relation to the vegetalizing factor from chicken embryos. Rouxs Arch Dev Biol 198:8–13
Gurdon JB, Fairman S, Mohun TJ, Brennan S (1985) Activation of muscle-specific actin genes inXenopus development by an induction between animal and vegetal cells of a blastula. Cell 41:913–922
Kimelman D, Kirschner M (1987) Synergistic induction of mesoderm by FGF and TGF-β and the identification of an mRNA coding for FGF in the earlyXenopus embryo. Cell 51: 869–877
Knöchel W, Born J, Hoppe P, Lopnow-Blinde B, Tiedemann H, Tiedemann H (1987) Mesoderm-inducing factors: their possible relationship to heparin-binding growth factors and transforming growth factor-β. Naturwissenschaften 74:604–606
Knöchel W, Tiedemann H, Tiedemann H (1989) Mesoderm induction by transforming growth factor: medium conditioned by TGF-treated ectoderm enhances the inducing activity. Naturwissenschaften 76:270–272
Mason AJ, Hayflick JS, Ling N, Esch F, Ueno N, Ying S-Y, Guillemin R, Niall H, Seeburg P (1985) Complementary DNA sequences of ovarian follicular fluid inhibin show precursor structure and homology with transforming growth factor-β. Nature 318:659–663
Murata M, Eto Y, Shibai H, Sakai M, Muramatsu M (1988) Erythroid differentiation factor is encoded by the same mRNA as that of the inhibin βA chain. Proc Natl Acad Sci USA 85:2434
Nakamura O, Takasaki H, Ishihara M (1971) Formation of the organizer from combinations of presumptive ectoderm and endoderm. Proc Jpn Acad 47:313–318
Nakamura T, Takio K, Eto Y, Shibai H, Titani K, Sugino H (1990) Activin-binding protein from rat ovary is follistatin. Science 247:836–838
Nakano H, Kinoshita K, Ishii K, Shibai H, Asashima M (1990) Activities of mesoderm-inducing factors secreted by mammalian cells in culture. Dev Growth Differ 32:165–170
Nieuwkoop PD, Faber J (1967) Normal table ifXenopus laevis (Daudin), second edition. North-Holland Publishing Company, Amsterdam
Nieuwkoop PD, Ubbels GA (1972) The formation of the mesoderm in urodelean amphibians: IV. Qualitative evidence for the purely “ectodermal” origin of the entire mesoderm and of the pharyngeal endoderm. Rouxs Arch Dev Biol 169:185–199
Nieuwkoop PD, Johnen AG, Albers B (1985) The epigenetic nature of early chordate development. Cambridge University Press, Cambridge
Paterno GD, Gillespie LL, Dixon MS, Slack JMW, Heath JK (1989) Mesoderm-inducing properties of INT-2 and kFGF: Two oncogene-encoded growth factors related to FGF. Development 106:79–83
Plessow S, Davids M, Born J, Hoppe P, Loppnow-Blinde B, Tiedemann H, Tiedemann H (1990) Isolation of a vegetalizing inducing factor after extraction with acid ethanol. Concentration-dependent inducing capacity of the factor. Cell Differ Dev 32:27–38
Robertson DM, Klein R, Vos FL de, McLachlan RI, Wettenhall RH, Hearn MTW, Burger HG, Krestser DM de (1987) The isolation of polypeptides with FSH suppressing activity from bovine follicular fluid which are structurally different to inhibin. Biochem Biophys Res Commun 149:744–749
Rosa F, Roberts AB, Danielpour D, Dart LL, Sporn MB, David IB (1988) Mesoderm induction in amphibians: the role of TGF βl2-like factors. Science 239:783–785
Slack JMW, Darlington BG, Heath JK, Godsave SF (1987) Mesoderm induction in earlyXenopus embryos by heparin-binding growth factors. Nature 326:197–200
Smith JC (1989) Mesoderm induction and mesoderm-inducing factors in early amphibian development. Development 105:665–677
Smith JC, Price BMJ, Van Nimmen K, Huylebroeck D (1990) Identification of a potentXenopus mesoderm-inducing factor as a homologue of activin A. Nature 345:729–731
Thomsen G, Woolf T, Whitman M, Sokol S, Vaughan J, Vale W, Melton DA (1990) Activins are expressed early inXenopus embryogenesis and can induce axial mesoderm and anterior structures. Cell 63:485–493
Tiedemann H (1990) Cellular and molecular aspects of embryonic induction. Zool Sci 7:171–186
Ueno N, Ling N, Ying S-Y, Esch F, Shimasaki S, Guillemin R (1987) Isolation and partial characterization of follistatin: a single-chain Mr. 35,000 monomeric protein that inhibits the release of follicle-stimulating hormone. Proc Natl Acad Sci USA 84:8282–8286
Vale W, Rivier J, Vaughan J, McClintock R, Corrigan A, Woo W, Karr D, Spiess J (1986) Purification and characterization of an FSH releasing protein from porcine ovarian fluid. Nature 321:776–779
van den Eijnden-Van Raaij AJM, van Zoelent EJJ, van Nimmen K, Koster CH, Snoek GT, Durston AJ, Huylebroeck D (1990) Activin-like factor from aXenopus laevis cell line responsible for mesoderm induction. Nature 345:732–734
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Asashima, M., Nakano, H., Uchiyama, H. et al. Follistatin inhibits the mesoderm-inducing activity of activin A and the vegetalizing factor from chicken embryo. Roux's Arch Dev Biol 200, 4–7 (1991). https://doi.org/10.1007/BF02457635
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02457635