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An alkaline-phosphatase staining method in avidin-biotin immunohistochemistry

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Summary

An avidin-biotin alkaline-phosphatase (ABAP) staining method has been developed for the labeling of tissue sections and cell smears. The introduction of alkaline phosphatase as a marker enzyme through an avidin bridge results in excellent immunocytochemical labeling of different antigens using poly- and monoclonal antibodies. This technique avoids problems with endogenous peroxidase activity that sometimes occur using peroxidase staining procedures. The introduction of a preformed avidin-biotin alkaline-phosphatase complex (ABAPC) makes the presented technique as simple to handle as the widely used avidin biotin-peroxidase complex method (ABC). The ABAPC technique could be combined with other enzymatic labelings for double immunoenzymatic staining.

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Authors and Affiliations

  1. Pathologisches Institut der Universität Würzburg, Luitpoldkrankenhaus, Josef-Schneider-Strasse 2, D-8700, Würzburg, Federal Republic of Germany

    W. Ormanns & R. Schäffer

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  1. W. Ormanns
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  2. R. Schäffer
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Supported by the Wilhelm-Sander-Stiftung

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Ormanns, W., Schäffer, R. An alkaline-phosphatase staining method in avidin-biotin immunohistochemistry. Histochemistry 82, 421–424 (1985). https://doi.org/10.1007/BF02450475

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  • DOI: https://doi.org/10.1007/BF02450475

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