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Chemical sterilization of nutrient media for plant cell cultures using diethylpyrocarbonate

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Biotechnology Techniques

Summary

Diethylpyrocarbonate (DPC), when added at about 1g/l to culture media is able to sterilize them. DPC kills all the contaminating microorganisms, and in contact with water it decomposes to ethanol and carbon dioxide in amounts not inhibiting the growth of plant cells. The plant cells cultivated on media treated by DPC did not show changes in their basic characteristics.

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Authors and Affiliations

  1. Dept. of Plant Tissue Cultures, Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo n. 2, 166 10, Prague 6

    T. Macek, J. Král, T. Vaněk & J. Blažek

  2. Dept. of Biochemistry and Microbiology, Faculty of Food and Biochem., Technology, ICT Prague, Technická 3, 166 28, Prague 6, Czech Republic

    M. Macková

Authors
  1. T. Macek
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  2. J. Král
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  3. T. Vaněk
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  4. J. Blažek
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  5. M. Macková
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Macek, T., Král, J., Vaněk, T. et al. Chemical sterilization of nutrient media for plant cell cultures using diethylpyrocarbonate. Biotechnol Tech 8, 885–888 (1994). https://doi.org/10.1007/BF02447733

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  • DOI: https://doi.org/10.1007/BF02447733

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