Summary
An improved method has been developed for the determination of fungal spore counts using a haemocytometer. The method uses a personal computer with a frame grabber card and commercial imaging software. Semi-automation using an iterative programming technique was implemented to count the spores produced byPhanerochaete chrysosporium. Rapid and reliable counts were achieved using image processing.
Similar content being viewed by others
Bibliography
Adams, H.L., and Thomas, C.R. (1988).Biotechnology and Bioengineering. 32, 707–712.
Allan, E.J., and Prosser, J.I. (1983).J. Gen. Microbiol. 129, 2029–2036.
Bumpus, J.A., and Aust, S.D. (1987).Appl. Environ. Microbiol. 53, 2001–2008.
Bumpus, J.A. (1989).Appl. Environ. Microbiol. 55, 154–158.
Glen, J.K., and Gold, M.H. (1983).Appl. Environ. Microbiol., 45, 1741–1747.
Hackett, W.F., Connors, W.J., Kirk, T.K., and Zeikus, J.G. (1977).Appl. Environ. Microbiol. 33, 43–51.
Humphreys, A.M., Matewele, P., Cunliffe, B., and Trinci, A.P.J. (1990).Mycol. Res. 94, 1046–1050.
Jähne, B. (1991).Digital Image Processing-Concepts, Algorithms and Scientific Applications. pp. 193–218, Berlin, Springer-Verlag.
Kirk, T.K., Schultz, E., Connors, W.J., Lorenz, L.F., and Zeikus, J.G. (1978).Arch. Microbiol. 117, 277–285.
Kirk, T.K., Croan, S., Tien, M., Murtugh, K.E. and Farrel, R.L. (1986).Enzyme Microb. Technol. 8, 27–32.
Lewandowski, G.A., Armenante, P.M., and Park, D. (1990).Water Res. 24, 75–82.
Lin, A.A., Nguyen, T., and Miller, W. (1991).Biotechnology Techniques. 5, 153–156.
Metz, B., de Bruijn, E.W., and van Suijdam, J.C. (1981).Biotechnol. Bioeng. 23, 149–162.
Morgan, P., Lewis, S.T., and Watkinson, R.J. (1991).Appl. Microbiol. Biotechnol. 34, 693–696.
Packer, H.L., Campero, B.M.T., and Thomas, C.R. (1988).Computer Applications in Fermentation Technology: Modelling and Control of Biotechnological Processes. N.M. Fish, R.I. Fox., and N.F. Thornhill, eds. pp. 23–35, London: Elsevier.
Ryan, T.P., and Bumpus, J.A. (1989).Appl. Microbiol. Biotechnol. 31, 302–307.
Venkatadri, R., and Irvine, R.L. (1990).Appl. Environ. Microbiol. 56, 2684–2691.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jones, C.L., Lonergan, G.T. & Mainwaring, D.E. The use of image analysis for spore counts of white-rot fungi. Biotechnol Tech 6, 417–422 (1992). https://doi.org/10.1007/BF02447481
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02447481