Abstract
Using a flexible piezoelectric film, the authors developed a simple system to determine noninvasively the heart rate of chicken embryos and hatchlings. The film was piezoelectric polyvinylidene fluoride (PVDF), which is sensitive enough to detect cardiogenic ballistic movements of the egg (ballistocardiogram (BCG)) and precordial movements of the hatchling attributable to cardiac contractions (apexcardiogram (ACG)). The BCG could be detected, during the second half of incubation, by placing the egg on the PVDF film on a soft substrate. The detected signal was found to be a measure of movement velocity. The ACG could be measured when the hatchling's chest wall made contact with the PVDF film installed in a box in which the hatchling was confined. The heart rate was counted from the lag time of autocorrelation calculated for a certain time segment (e.g. 2s) of the BCG and ACG recordings.
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References
Cain, J. R., Abbott, U. K. andRogallo, V. L. (1967) Heart rate of the developing chick embryo.Proc. Soc. Exp. Biol. Med.,26, 507–510.
Dario, P., De Rossi, D. E., Bedini, R., Francesconi, R. andTrivella, M. G. (1984) PVF2 catheter-tip transducer for pressure sound and flow measurements.Ferroelectrics,60, 149–162.
Hasegawa, J., Kobayashi, K. andKondou, S. (1991) An analysis of the influences of the environmental factors on the embryonic heart rate variability using egg.Med. & Biol. Eng. & Comput.,29, Suppl. 2, 16th World Congr. on Med. Physics & Biomed. Eng., 16th Int. Conf. on Med. & Biol. Eng. and 9th Int. Conf. on Med. Physics, Kyoto, Japan, 7th–12th July 1991, 1294.
Hashimoto, Y., Narita, T. andTazawa, H. (1991) Cardiogenic ballistograms of chicken eggs: comparison of measurements.Med. & Biol. Eng. & Comput.,29, 393–397.
Kawai, H. (1969) Piezoelectricity of polyvinylidenefluoride.Jpn. J. Appl. Phys.,8, 975–976.
Kobayashi, K. andYasuda, T. (1981) An application of PVDF-film to medical transducers. InMedical applications of piezoelectric polymers.Galletti, P. M., De Rossi, D. E. andDe Reggi, A. S. (Eds.), Gordon & Breach Sci. Publ., 99–105.
Sessler, G. M. (1981) Piezoelectricity in polyvinylidenefluoride.J. Acoust. Soc. Am.,70, 1596–1608.
Siivola, J. (1989) New noninvasive piezoelectric transducer for recording of respiration, heart rate and body movements.Med. & Biol. Eng. & Comput.,27, 423–424.
Suzuki, Y., Musashi, H. andTazawa, H. (1989) Noninvasive heart rate monitoring system for avian embryos based on the ballistocardiogram. —Ibid.,27, 399–404.
Tazawa, H. (1980) Adverse effect of failure to turn the avian egg on the embryo oxygen exchange.Respirat. Physiol.,41, 137–142.
Tazawa, H. andJohansen, K. (1987) Comparative model analysis of central shunts in vertebrate cardiovascular systems.Comput. Biochem. Physiol.,86A, 595–607.
Tazawa, H., Hiraguchi, T., Asakura, T., Fujii, H. andWhittow, G. C. (1989a) Noncontact measurements of avian embryo heart rate by means of the laser speckle: comparison with contact measurements.Med. & Biol. Eng. & Comput.,27, 580–586.
Tazawa, H., Suzuki, Y. andMusashi, H. (1989b) Simultaneous acquisition of ECG, BCG and blood pressure from chick embryos in the egg.J. Appl. Physiol.,67, 478–483.
Tazawa, H., Kuroda, O. andWhittow, G. C. (1991a) Noninvasive determination of embryonic heart rate during hatching in the Brown Noddy (Anous stolidus).Auk,108, 594–601.
Tazawa, H., Hiraguchi, T., Kuroda, O., Tullett, S. G. andDeeming, O. C. (1991b) Embryonic heart rate during development of domesticated birds.Physiol. Zool.,64, 1002–1022.
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Tazawa, H., Hashimoto, Y., Takami, M. et al. Simple, noninvasive system for measuring the heart rate of avian embryos and hatchlings by means of a piezoelectric film. Med. Biol. Eng. Comput. 31, 129–134 (1993). https://doi.org/10.1007/BF02446670
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DOI: https://doi.org/10.1007/BF02446670