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Vanadium compounds promote the induction of morphological transformation of hamster embryo cells with no effect on gap junctional cell communication

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Abstract

Vanadium compounds were found to promote the induction of morphological transformation of hamster embryo cells. Exposure of the cells to Na−O-vanadate, vanadin (V) oxide or vanadin (IV) oxide sulfate following pre-exposure to a low concentration of benzo[a]pyrene, potentiated the induction of transformed colonies similar to 12-O-tetradecanoylphorbol-13-acetate. Unlike this phorbol ester, vanadium compounds did not inhibit intercellular communication, or activate protein kinase C. Nor did vanadate influence the reoccurrence of communication after removal of a communication blocking phorbol ester. On the other hand, vanadate showed strong synergism with the phorbol ester on induction of transformed morphology in the phorbol ester sensitive cell line BPNi. This suggests that vanadium and tumor promoting phorbol esters mediate their effect on the induction of morphological transformation of hamster embryo cells through different mechanisms.

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Abbreviations

BaP:

benzo[a]pyrene

DMEM:

Dulbecco's modified Eagle's medium

EGF:

epidermal growth factor

SHE:

Syrian hamster embryo

TPA:

12-O-tetradecanoyl-phorbol-13-acetate

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Authors and Affiliations

  1. Laboratory for Environmental and Occupational Cancer Institute for Cancer Research, The Norwegian Radium Hospital, N-0310, Oslo 3, Norway

    Edgar Rivedal, Lars E. Roseng & Tore Sanner

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  1. Edgar Rivedal
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  2. Lars E. Roseng
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  3. Tore Sanner
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Rivedal, E., Roseng, L.E. & Sanner, T. Vanadium compounds promote the induction of morphological transformation of hamster embryo cells with no effect on gap junctional cell communication. Cell Biol Toxicol 6, 303–314 (1990). https://doi.org/10.1007/BF02443805

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