Abstract
We describe new primers for the detection of the STR polymorphism at the CSF1PO locus. These primers have been designed to produce shorter amplicons (150–182 bp) than the primers in standard use (295–327 bp). The reliability of the new primers for CSF1PO typing has been demonstrated by testing on known samples and by sequence analysis. These primers are superior to the original primers with regard to electrophoretic resolution and utility for typing of severely degraded DNA.
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References
Alford RL, Hammond HA, Coto I, Caskey CT (1994) Rapid and efficient resolution of parentage by amplification of short tandem, repeats. Am J Hum Genet 55:190–195
Clark JM (1988) Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases. Nucleic Acid Res 16:9677–9686
Gill P, Ivanov PL, Kimpton C, Piercy R, Benson N, Tully G, Evett I, Hagelberg E, Sullivan, K (1994) Identification of the remains of the Romanov family by DNA analysis. Nat Genet 6:130–135
Hammond HA, Jin L, Zhong Y, Caskey CT, Chakraborty R (1994) Evaluation of 13 short tandem repeat loci for use in personal identification applications. Am J Hum Genet 55:175–189
Hochmeister MN, Budowle B, Borer UV, Rudin O, Bohnert M, Dirnhofer R (1995) Confirmation of the identity of human skeletal remains using multiplex PCR amplification and typing kits. J Forensic Sci 40:701–705
Hochmeister MN, Budowle B, Schumm JW, Sprecher CJ, Borer UV, Dirnhofer R (1995) Swiss population data and forensic efficiency values on 3 tetrameric short tandem repeat loci-HUMTH01, TPOX and CSF1PO- derived using a STR multiplex system. Int J Legal Med 107:246–249
Hochmeister MN, Budowle B, Eisenberg A, Borer UV, Dirnhofer R (1996) Using multiplex PCR amplification and typing kits for the analysis of DNA evidence in a serial killer case. J Forensic Sci 41:155–162
Huang NE, Schumm J, Budowle B (1995) Chinese population data on three tetrameric short tandem repeat loci-HUMTH01, TPOX and CSF1PO-derived using multiplex PCR and manual typing. Forensic Sci Int 71:131–136
Kimpton CP, Gill P, Walton A, Urquhart A, Millican ES, Adams M (1993) Automated DNA profiling, employing multiplex amplification of short tandem repeat loci. PCR Methods Appl 3:13–22
Klintschar M (1995) Validation of the system FES/FPS for forensic purpose in an Austrian population sample. Int J Legal Med 108:162–164
Lygo JF, Johnson PE, Holdaway DJ, Woodroff S, Whitaker JP, Clayton TM, Kimpton CP, Gill P (1994) The validation of short tandem repeat (STR) loci for use in forensic casework. Int J Legal Med 107:77–89
Puers C, Hammond HA, Jin L, Caskey CT, Schumm JW (1993) Identification of repeat sequence heterogeneity at the polymorphic short tandem repeat locus HUMTH01[AATG]n and reassignment of alleles in population analysis by using a locus-specific allelic ladder. Am J Hum Genet 53:953–958
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning, a laboratory manual, 2nd edn Cold Spring Harbor Laboratory Press, Cold Spring Harbor
Wang Y, Wallin JM, Ju J, Sensabaugh GF, Mathies RA (1996) High-resolution capillary array electrophoretic sizing of multiplexed short tandem repeat loci using energy-transfer fluorescent primers. Electrophoresis 17:1485–1490
Whitaker JP, Clayton TM, Urquhart AJ, Millican ES, Downes TJ, Kimpton CP, Gill P (1995) Short tandem repeat typing of bodies from a mass disaster: high success rate and characteristic amplification patterns in highly degraded sampley. Biotechniques 18:670–677
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Yoshida, K., Sekiguchi, K., Kasai, K. et al. Evaluation of new primers for CSF1PO. Int J Leg Med 110, 36–38 (1997). https://doi.org/10.1007/BF02441025
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DOI: https://doi.org/10.1007/BF02441025