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Effect of background fluorophores on the NADH fluorescence probe signal

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Biotechnology Techniques

Summary

Analysis of the errors caused by a drift in background fluorescence is performed for a fluorescence probe operated in a backscatter configuration. In some cases, significant errors can result if changes in background fluorophores during the course of fermentation are not accounted for. It is shown that the probe's sensitivity to background fluorophores must be considered to calibrate a fluorescence probe properly.

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Authors and Affiliations

  1. Department of Chemical Engineering, University of Maryland, 20742, College Park, Maryland, U.S.A.

    Nam Sun Wang & Michael B. Simmons

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  1. Nam Sun Wang
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  2. Michael B. Simmons
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Wang, N.S., Simmons, M.B. Effect of background fluorophores on the NADH fluorescence probe signal. Biotechnol Tech 5, 241–246 (1991). https://doi.org/10.1007/BF02438655

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  • DOI: https://doi.org/10.1007/BF02438655

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