Skip to main content
SpringerLink
Log in

Susceptibility of mouse liver DNA to digestion by S1 nuclease: Absence of age-related change

  • Brief Communications
  • Published:
AGE Aims and scope Submit manuscript

Abstract

The sensitivity of native liver DNA to S1 nuclease did not increase with age in C57BL/6J mice up to 30 mo (the average lifespan). These data fail to confirm previous reports implying an increase of single-stranded regions in DNA from old mice.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Chetsanga, C.J., Boyd, V., Peterson, L., and Rusholow, K.: Single-stranded regions in DNA of old mice. Nature 253:130–131, 1975.

    Article  PubMed  CAS  Google Scholar 

  2. Chetsanga, C.J., Tuttle, M., and Jacoboni, A.: Changes in structural integrity of heart DNA from aging mice. Life Sci. 18:1405–1412, 1976.

    Article  PubMed  CAS  Google Scholar 

  3. Chetsanga, C.J., Tuttle, M., Jacoboni, A., and Johnson, C.: Age-associated structural alterations in senescent mouse brain DNA. Biochim. Biophys. Acta 474:180–187, 1977.

    PubMed  CAS  Google Scholar 

  4. Finch, C.E.: The comparative biology of senescence: some evolutionary and developmental considerations. Animal Models for Biomedical Research, IV. Proc. Nat. Acad. Sci. (USA), 1971, p 47.

  5. Dean, R.G. and Cutler, R.G.: Absence of significant age-dependent increase of single-stranded DNA extracted from mouse liver nuclei. Exp. Geront. 13:287–292, 1978.

    Article  CAS  Google Scholar 

  6. Rice, N.R.: Susceptibility of native, reassociated, and mismatched DNA to S1 nuclease. Carnegie Inst. Yr. Book 73:1097–1098, 1974.

    Google Scholar 

  7. Finch, C.E.: Catecholamine metabolism in the brains of ageing male mice. Brain Res. 52:261–276, 1973.

    Article  PubMed  CAS  Google Scholar 

  8. Chauveau, J., Moule, Y., Rouiller, C.: Isolation of pure and unaltered liver cell nuclei; morphology and biochemical composition. Exp. Cell. Res. 11:317–321, 1956.

    Article  PubMed  CAS  Google Scholar 

  9. Britten, R.J., Graham, D.E., Neufeld, B.R.: Analysis of repeating DNA sequences by reassociation. Methods in Enzymology, 29E. New York, Academic Press, 1974, p. 368.

    Google Scholar 

  10. KapLan, B.B., Schachter, B.S., Osterburg, H.H., de Vellis, J.S., and Finch, C.E.: Sequence complexity of polyadenylated RNA obtained from rat brain regions and cultured cells of neural origin. Biochemistry 17: 5516–5524, 1978.

    Article  PubMed  CAS  Google Scholar 

  11. Vogt, V.M.: Purification and further properties of single-stranded-specific nuclease from Aspergillus oryzae. Eur. J. Biochem. 33:192–200, 1973.

    Article  PubMed  CAS  Google Scholar 

  12. Hutton, J.R. and Wetmur, J.G.: Length dependence of the complexity of mouse satellite DNA. Biochem. Biophys. Res. Comm. 52:1148–1155, 1973.

    Article  PubMed  CAS  Google Scholar 

  13. Smith, M.J., Britten, R.J. and Davidson, E.H.: Studies on nucleic acid reassociation kinetics: Reactivity of single-stranded tails in DNA-DNA renaturation. Proc. Nat. Acad. Sci. (USA) 72:4805–4809, 1975.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Andrus Gerontology Center, University of Southern California, University Park, Los Angeles, California, 90007

    Caleb E. Finch

  2. Department of Molecular Biology, University of Southern California, University Park, Los Angeles, California, 90007

    Caleb E. Finch

Authors
  1. Caleb E. Finch
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Finch, C.E. Susceptibility of mouse liver DNA to digestion by S1 nuclease: Absence of age-related change. AGE 2, 45–46 (1979). https://doi.org/10.1007/BF02432255

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02432255

Keywords

Search

Navigation